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01 November 2018 : Laboratory Research  

Interleukin-22 Promotes Osteosarcoma Cell Proliferation and Invasion via STAT3 Activation

Panpan Li1BCEF, Xin Shi2BCDE, Yonghui Xu1BD, Binggang Zhong3BC, Yu Lu4BC, Yong Sun1ABCDEFG*

DOI: 10.12659/MSM.910962

Med Sci Monit 2018; 24: LBR7802-7808

Abstract

BACKGROUND: Interleukin-22 (IL-22) is one of the cytokines secreted by T-helper 17 (Th17) cells. It belongs to the IL-10 cytokine family and influences a variety of immune reactions. Studies have indicated that IL-22 can promote cancer progression and metastases. However, the function of IL-22 in osteosarcoma (OS) remains unclear.

MATERIAL AND METHODS: In this study, the expression of IL-22 in the OS cell line was detected by qRT-PCR. The role of IL-22 in proliferation and invasion in OS cells was tested by MTT and Transwell assays. The protein expression of STAT3, phospho-STAT3, AKT, and phospho-AKT was detected by Western blot analysis.

RESULTS: The results showed that IL-22 was upregulated in OS cells. IL-22 dose-independently promoted OS cells proliferation and invasion, which could be reversed by IL-22 antibody or STAT3 siRNA. Furthermore, IL-22 exposure of OS cells resulted in dose-independently increased levels of phosphorylated STAT3 protein kinases. Interestingly, IL-22 did not influence the expression of phosphorylated AKT.

CONCLUSIONS: These results suggest that IL-22 promotes OS cells proliferation and invasion and its effect is mediated by activation of the STAT3 pathway. These findings demonstrate that IL-22 may serve as a promising molecular biomarker for diagnosis and therapy for OS patients.

Keywords: STAT3 Transcription Factor

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750