Logo MSM

20 November 2018 : Laboratory Research

Over-Expression of ATPase II Alleviates Ethanol-Induced Hepatocyte Injury in HL-7702 Cells

Qing Li1ABC, Yan Yang1ABC, Ying Liu1EF*

Med Sci Monit 2018; 24: LBR8372-8382

Abstract

BACKGROUND: Excessive alcohol consumption can cause hepatocellular injury. ATPase II (ATP8A1) can display an ATP-dependent phospholipid translocase activity. However, the function of ATP8A1 in hepatocyte injury is still unclear. In the present study we explored the effect of ATP8A1 on ethanol-induced hepatocyte injury.

MATERIAL AND METHODS: A human hepatocyte strain, HL-7702, was pretreated by ethanol with gradient concentration for 2, 4, 8, and 12 h, and were then divided into 6 groups after the cells were transfected. We detected cell viability by use of the Cell Counting Kit-8 (CCK-8) assay. Reactive oxygen species (ROS), apoptosis rate, and mitochondrial membrane potential (MMP) were measured using flow cytometry. We used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot to measure the mRNA and protein expression, respectively.

RESULTS: Ethanol inhibited the viability of HL-7702 cells and suppressed the expression of ATP8A1 in dose- and time-dependent manners. Furthermore, over-expression of ATP8A1 reduced the level of ROS and the apoptosis rate and recovered the MMP. Additionally, over-expressed ATP8A1 regulated the protein and mRNA levels of apoptosis-related molecules. Moreover, over-expression of ATP8A1 enhanced the phosphorylation of phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt).

CONCLUSIONS: Over-expression of ATP8A1 alleviated ethanol-induced hepatocyte injury. Moreover, the PI3K/Akt signaling pathway appears to participate in inhibition of ethanol-induced hepatocyte apoptosis and may provide a candidate target for the treatment of alcoholic liver diseases (ALD).

Keywords: Arsenite Transporting ATPases, Hepatocytes, phosphatidylinositol 3-kinase

0 Comments

This Week Editorial

02 August 2021 : Editorial

Editorial: The National COVID Cohort Collaborative Consortium Combines Population Data with Machine Learning to Evaluate and Predict Risk Factors for the Severity of COVID-19

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.934171

Med Sci Monit 2021; 27:e934171

Coronavirus/Covid 19

30 July 2021 : Clinical Research

Rural and Female Patients with Old Myocardial Infarction Lacked Knowledge and Preventive Measures During th...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.928512  

16 July 2021 : Review article

Silent Hypoxemia in Patients with COVID-19 Pneumonia: A Review

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.930776  

09 July 2021 : Database Analysis

A Simple Clinical Prediction Tool for COVID-19 in Primary Care with Epidemiology: Temperature-Leukocytes-CT...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.931467  

07 July 2021 : Database Analysis

Chest Radiological Findings and Clinical Characteristics of Laboratory-Confirmed COVID-19 Patients from Sau...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.932441  

In Press

03 Aug 2021 : Clinical Research

Comparison of Outcomes Following TiRobot-Assisted Sacroiliac Screw Fixation with Bone Grafting and Traditio...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.932724  

02 Aug 2021 : Database Analysis

RNA N6-Methyladenosine Patterns in Hepatocellular Carcinoma Reveal a Distinct Immune Infiltration Landscape...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.930994  

02 Aug 2021 : Clinical Research

Vitamin D Receptor Is a Sepsis-Susceptibility Gene in Chinese Children

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.932518  

30 Jul 2021 : Clinical Research

Rural and Female Patients with Old Myocardial Infarction Lacked Knowledge and Preventive Measures During th...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.928512  

Most Viewed

20 Mar 2020 : Clinical Research

Social Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...

DOI :10.12659/MSM.923921

Med Sci Monit 2020; 26:e923921

15 Apr 2020 : Clinical Research

Psychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...

DOI :10.12659/MSM.924171

Med Sci Monit 2020; 26:e924171

05 May 2020 : Review article

An Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development

DOI :10.12659/MSM.924700

Med Sci Monit 2020; 26:e924700

26 Apr 2020 : Clinical Research

Comparison of Prevalence and Associated Factors of Anxiety and Depression Among People Affected by versus P...

DOI :10.12659/MSM.924609

Med Sci Monit 2020; 26:e924609

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750