Jinfu’an Decoction Inhibits Invasion and Metastasis in Human Lung Cancer Cells (H1650) via p120ctn-Mediated Induction and Kaiso
Zhe Sun, Yang Cao, Guangyun Hu, Jiuda Zhao, Ming Chen, Sisi Wang, Zengjie Ye, Hongyu Chen, Wenping Wang, Ya’nan Wang
The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China (mainland)
Med Sci Monit 2018; 24: CLR2878-2886
Previous research showed that Jin-Fu-An decoction has a significant effect on lung cancer. However, it remains unclear whether p120ctn and its transcription factor Kaiso play a role in lung cancer cell proliferation, adhesion, migration, and metastasis.
MATERIAL AND METHODS: Proliferation inhibition was detected by CCK-8 assay. The migration and invasion were detected using Transwell assay. The location and expression of p120ctn and Kaiso were monitored by immunofluorescence staining. The expression changes of p120ctn, its isoform 1A, its S288 phosphorylation, and Kaiso were measured by Western blot assay.
RESULTS: The lung cancer cell line H1650 administered Jin-Fu-An decoction had significantly reduced the growth in dose-dependent and time-dependent manners. Migration and metastasis were significantly inhibited by application of Jin-Fu-An decoction in a dose-dependent manner. Additionally, Jin-Fu-An decoction decreased the expressions of p120ctn, its isoform 1A, and its S288 phosphorylation, but the protein level of Kaiso was elevated.
CONCLUSIONS: Jin-Fu-An decoction inhibits the proliferation, adhesion, migration, and metastasis though down-regulation of p120ctn or its isoform 1A expression, mediating the up-regulation of Kaiso. The underlying mechanism of Jin-Fu-An decoction might involve targeting the lower expression of p120ctn S288 phosphorylation, which suggests that Jin-Fu-An decoction may be a potential therapeutic measure as prevention and control of recurrence and metastasis of lung cancer.
Keywords: Chinese Medicinal Formula, Invasion and Metastasis, Jinfu’an Decoction, lung cancer, p120ctn and Kasio