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28 March 2018 : Laboratory Research  

MicroRNA Expression Profiling of Bone Marrow Mesenchymal Stem Cells in Steroid-Induced Osteonecrosis of the Femoral Head Associated with Osteogenesis

Ao Wang1ABCDEFG, Ming Ren1ABCDEFG, Yang Song1ABCDEFG, Xiaonan Wang1ABCF, Qingyu Wang1ABD, Qiwei Yang1ABC, He Liu1CDEF, Zhenwu Du1ABF, Guizhen Zhang1ABD, Jincheng Wang1ABCDEFG*

DOI: 10.12659/MSM.909655

Med Sci Monit 2018; 24: LBR1813-1825

Abstract

BACKGROUND: Steroid-induced osteonecrosis of the femoral head (SONFH) is a common orthopedic disease associated with the application of glucocorticoid (GC). In this study, we detected the microRNAs (miRNAs) differentially expressed in bone marrow mesenchymal stem cells (BMSCs) from SONFH patients, and target gene predictions were performed, and the functions of the target genes was verified.

MATERIAL AND METHODS: BMSCs collected from patients with SONFH and femoral neck fracture (FNF) constituted the SONFH group (n=3) and FNF (control) group (n=3), respectively. MiRNA microarray analysis was utilized to detect the differentially expressed miRNAs, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the microarray results. The target genes and functions of the differentially expressed miRNAs were analyzed using a bioinformatics database.

RESULTS: The microarray results revealed that compared with the control group, 22 miRNAs were identified differentially expressed in the SONFH group, with 17 upregulated and 5 downregulated. Further qRT-PCR validation of differentially expressed miRNAs confirmed that hsa-miR-601, hsa-miR-452-3p, hsa-miR-647, and hsa-miR-516b-5p were significantly increased, whereas hsa-miR-122-3p was significantly decreased. During osteogenic differentiation, hsa-miR-601, hsa-miR-452-3p, hsa-miR-647, hsa-miR-516b-5p, and hsa-miR-127-5p were significantly downregulated, whereas hsa-miR-122-3p was significantly upregulated, and miRNAs showed a converse tendency during adipogenic differentiation.

CONCLUSIONS: Six miRNAs associated with osteogenic and adipogenic differentiation were identified differentially expressed in the BMSCs of SONFH patients; these miRNAs may serve as novel biomarkers or therapeutic targets for SONFH.

Keywords: Bone Diseases, Femur Head Necrosis, Glucocorticoids, mesenchymal stromal cells, Osteogenesis

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750