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28 May 2018 : Laboratory Research  

Overexpression of the Ubiquilin-4 (UBQLN4) is Associated with Cell Cycle Arrest and Apoptosis in Human Normal Gastric Epithelial Cell Lines GES-1 Cells by Activation of the ERK Signaling Pathway

Shengkai Huang1234AEF, Xin Dong125AEF, Jia Wang6BD, Jie Ding7BDE, Yan Li235BD, Dongdong Li235BD, Hong Lin235BD, Wenjie Wang235BD, Mei Zhao235E, Qing Chang8AF*, Ning Zhou9AF, Wei Cui1AE, Changzhi Huang234AEFG

DOI: 10.12659/MSM.909621

Med Sci Monit 2018; 24: LBR3564-3570

Abstract

BACKGROUND: Ubiquilin-4 (UBQLN4) is a component of the ubiquitin-proteasome system and regulates the degradation of many proteins implicated in pathological conditions. The aim of this study was to determine the role of UBQLN4 in regulating the proliferation and survival of the normal gastric epithelial cell line GES-1.

MATERIAL AND METHODS: We constructed GES-1 lines stably overexpressing UBQLN4 by lentiviral infection. Cell proliferation, apoptosis, and the cell cycle were analyzed using the MTT assay and flow cytometric assays. Phosphorylation of ERK, JNK, p38, and expression of cyclin D1 were detected by western blot analysis.

RESULTS: Overexpression of UBQLN4 significantly reduced proliferation and induced G2/M phase arrest and apoptosis in GES-1 cells. Moreover, upregulation of UBQLN4 increased the expression of cyclin D1 and phosphorylated ERK, but not JNK or p38.

CONCLUSIONS: These data suggest that UBQLN4 may induce cell cycle arrest and apoptosis via activation of the ERK pathway and upregulation of cyclin D1 in GES-1 cells.

Keywords: Ubiquitins

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750