Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

08 June 2018 : Laboratory Research  

Upregulation of KIF26B, Cell Migration and Proliferation of Human Ovarian Cancer Cell Lines In Vitro, and Patient Outcomes from Human Bioinformatic Analysis

Xingshuang Yang1BCDE, Lan Zhang2ABCDE, Lei Xie3ABCDEF*

DOI: 10.12659/MSM.907889

Med Sci Monit 2018; 24: LBR3863-3872

Abstract

BACKGROUND: The aim of this study was to assess the expression profile of the KIF26B gene, its effects on ovarian cancer cell behavior in vitro, the clinical prognostic value of expression of the KIF26B gene and associated signaling pathways, from bioinformatics data analysis.

MATERIAL AND METHODS: The Cancer Genome Atlas-Ovarian Cancer (TCGA-OV) database and the online Kaplan-Meier plotter for ovarian cancer were analyzed. Human ovarian cancer cell lines A2780 and SKOV3 were used to study the in vitro effects of KIF26B gene expression on cell proliferation and cell invasion. Genes that interacted with, co-localized to, and were co-expressed with the KIF26B gene (Pearson’s r ≥0.6) were identified and underwent Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

RESULTS: Increased expression of the KIF26B gene increased the proliferation and migration of A2780 and SKOV3 cells in vitro. KIF26B protein expression was upregulated in ovarian cancer tissue and was associated with lymphatic and venous invasion. TCGA-OV data analysis showed that increased expression of the KIF26B gene was significantly associated with reduced 3-year, 5-year, and 10-year overall survival (OS), progression-free survival (PFS), and post-progression survival (PPS). The genes that interacted with, co-localized to, and were co-expressed with KIF26B were enriched in several KEGG pathways; upregulation of the KIF26B gene was associated with TGF-β signaling pathway.

CONCLUSIONS: Upregulation of KIF26B enhanced proliferation and migration of ovarian cancer cells in vitro. Bioinformatics analysis supported the association between increased expression of the KIF26B gene and reduced clinical outcome in patients with ovarian carcinoma.

Keywords: Prognosis

0 Comments

Editorial

01 December 2021 : Editorial  

Editorial: SARS-CoV-2 Vaccine Responses and Breakthrough COVID-19

Dinah V. Parums
Scientific Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.935624

Med Sci Monit 2021; 27:e935624

SARS-CoV-2/COVID-19

03 December 2021 : Laboratory Research  

Influenza and Influenza-Like Respiratory Virus Infections in Children During the 2019/20 Influenza Season a...

Med Sci Monit In Press; DOI: 10.12659/MSM.934862  

08 November 2021 : Database Analysis  

Virtual Screening and Molecular Docking to Study the Mechanism of Chinese Medicines in the Treatment of Cor...

Med Sci Monit In Press; DOI: 10.12659/MSM.934102  

01 December 2021 : Editorial  

Editorial: SARS-CoV-2 Vaccine Responses and Breakthrough COVID-19

Med Sci Monit 2021; 27:e935624

In Press

03 Dec 2021 : Clinical Research  

A Retrospective Single-Center Study of 23 Patients to Compare Gait Before and After Total Hip Arthroplasty ...

Med Sci Monit In Press; DOI: 10.12659/MSM.934558  

03 Dec 2021 : Laboratory Research  

Influenza and Influenza-Like Respiratory Virus Infections in Children During the 2019/20 Influenza Season a...

Med Sci Monit In Press; DOI: 10.12659/MSM.934862  

02 Dec 2021 : Clinical Research  

Comparison of Outcomes Following Neuronavigation-Assisted Aspiration and Thrombolysis Using Single and Mult...

Med Sci Monit In Press; DOI: 10.12659/MSM.934935  

02 Dec 2021 : Clinical Research  

Serum Levels of N-Terminal Pro-Brain Natriuretic Peptide in Gestational Hypertension, Mild Preeclampsia, an...

Med Sci Monit In Press; DOI: 10.12659/MSM.934285  

Most Viewed Current Articles

20 Mar 2020 : Clinical Research  

Social Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...

DOI :10.12659/MSM.923921

Med Sci Monit 2020; 26:e923921

15 Apr 2020 : Clinical Research  

Psychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...

DOI :10.12659/MSM.924171

Med Sci Monit 2020; 26:e924171

05 May 2020 : Review article  

An Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development

DOI :10.12659/MSM.924700

Med Sci Monit 2020; 26:e924700

26 Apr 2020 : Clinical Research  

Comparison of Prevalence and Associated Factors of Anxiety and Depression Among People Affected by versus P...

DOI :10.12659/MSM.924609

Med Sci Monit 2020; 26:e924609

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750