The Impact of EGFR Gene Polymorphisms on the Risk of Alzheimer’s Disease in a Chinese Han Population: A Case-Controlled Study
Xiuhong Chen, Changhai Wang, Shuangbao Zhou, Xueyong Li, Lan Wu
School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing, China (mainland)
Med Sci Monit 2018; 24:5035-5040
The aim of this study was to investigate the association between polymorphisms of the epidermal growth factor receptor (EGFR) gene with the risk of Alzheimer’s disease (AD) in a Chinese Han population.
MATERIAL AND METHODS: A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to genotype 139 patients with AD and 152 healthy control individuals. The Hardy-Weinberg equilibrium (HWE) was analyzed using the chi-squared (χ²) test, and genotype and allele frequencies were compared between the two population groups, using the χ² test. The odds ratios (ORs) and corresponding 95% confidence intervals (CI) were calculated to express the degree of risk of AD resulting from polymorphisms in the EGFR gene. Linkage disequilibrium among EGFR polymorphisms was analyzed using the Haploview bioinformatics software.
RESULTS: The CC genotype and C allele frequencies of rs730437 were significantly lower in patients with AD compared with the controls (P=0.037), indicating that rs730437 was associated with a reduced risk of AD (CC vs. AA: OR=0.446, 95% CI=0.207–0.960) (C vs. A: OR=0.702, 95% CI=0.502–0.980). The presence of the TT genotype of rs1468727 significantly reduced the risk of AD (P=0.003; OR=0.333, 95% CI=0.160–0.691), and T allele carriers of rs1468727 had a 0.605-fold increased risk of AD. Haplotype A-C-C was significantly correlated with an increased risk of AD (OR=1.922, 95% CI=1.130–3.269).
CONCLUSIONS: In a Han Chinese population, EGFR gene polymorphisms, rs730437 and rs1468727 and haplotype A-C-C were shown to be possible protective factors for the development of AD.
Keywords: Alzheimer Disease, Haplotypes, Polymorphism, Genetic, Receptor, Epidermal Growth Factor