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20 February 2018 : Laboratory Research  

Paeoniflorin Inhibits Receptor Activator for Nuclear Factor κB (RANK) Ligand-Induced Osteoclast Differentiation In Vitro and Particle-Induced Osteolysis In Vivo

Zhuokai Li1ABCD, De Li1ABCD, Xiaodong Chen1EF*

DOI: 10.12659/MSM.907739

Med Sci Monit 2018; 24: LBR1044-1053


BACKGROUND: Paeoniflorin (PF), a glucoside isolated from the dried root of Paeonia lactiflora Pall, has been reported to have a number of pharmacological properties, including immunity-regulation, anticancer activities, and neuroprotective effect. However, PF’s pharmacological role in bone disorder has been seldom reported. Hence, this study was designed to investigate the effects of PF on osteoclast differentiation and osteolysis diseases.

MATERIAL AND METHODS: The bone marrow macrophages were isolated from C57BL/6 mice and incubated with RANK ligand (RANKL) and various concentrations of PF. After 5 days of incubation, tartrate-resistant acid phosphatase (+) cells and bone resorption pits were counted. Effects of PF on expression of osteoclast-specific protein and gene were investigated via Western blot, q-PCR, and immunofluorescence assay. The osteoprotective effect of PF in vivo was evaluated in a calvarial osteolysis model via micro-CT scan and histological stain.

RESULTS: In vitro, PF intervention inhibited osteoclast formation and resorption activity. PF also impaired RANKL-induced NF-κB phosphorylation and immigration to the nucleus. PF suppressed osteoclast-marker protein and gene expression. In vivo, PF inhibited cobalt-chromium-molybdenum alloy particle-induced osteolysis and reduced osteoclast number in tissue slice.

CONCLUSIONS: PF is a potential agent against osteolysis-related diseases caused by excessive osteoclast activity.

Keywords: Osteoclasts, Prosthesis Failure, Receptor Activator of Nuclear Factor-kappa B



01 December 2021 : Editorial  

Editorial: SARS-CoV-2 Vaccine Responses and Breakthrough COVID-19

Dinah V. Parums
Scientific Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.935624

Med Sci Monit 2021; 27:e935624


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26 Nov 2021 : Clinical Research  

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Med Sci Monit In Press; DOI: 10.12659/MSM.934007  

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750