Association of Single-Nucleotide Polymorphisms of CD44 Gene with Susceptibility to Breast Cancer in Chinese Women
Xiao Lin, Xiaojing You, Xuezhen Cao, Shenghua Pan
Department of Thyroid and Breast Surgery, Ruian People's Hospital of Zhejiang Province, Wenzhou, Zhejiang, China (mainland)
Med Sci Monit 2018; 24:3077-3083
This study aimed to evaluate the association of CD44 gene single-nucleotide polymorphisms with susceptibility to breast cancer.
MATERIAL AND METHODS: This case-control study included 242 breast cancer patients and 252 normal people without disease. The single-nucleotide polymorphisms of the CD44 gene in the 2 groups were genotyped by PCR-LDR method. The OR and its 95% CI was calculated by chi-square test and logistic regression analysis. The construction of haplotypes and their interaction analysis with relevant factors were carried out by SHEsis and SNPStats online.
RESULTS: The genotype distribution of CC and CT, CC and CC+CT, and CC+CT and TT in rs13347 showed a significant difference between cases and controls, and the difference in distribution of alleles C and T was statistically significant. The genotype and alleles distribution of rs4756195 and rs8193 showed no statistically significant difference (P>0.05). The haplotypes distribution of CAC, CGT, TAC, and TGT showed a significant difference between the 2 groups (P<0.05). The results of analysis of haplotypes and their interactions with relevant factors showed that breast cancer risk in the PR-negative group was significantly higher than that in the PR-positive group (P=0.016). We found an interaction between haplotypes and PR status.
CONCLUSIONS: The genotypes CT, CT+TT, TT, and allele T in rs13347 may be risk factors for breast cancer. The haplotype CAC may be a protective factor against breast cancer, and CGT, TAC, and TGT may be risk factors for breast cancer. The PR status interacts with CD44 gene SNP.
Keywords: Antigens, CD44, Polymorphism, Single Nucleotide, Triple Negative Breast Neoplasms