Logo MSM

28 October 2017 : Laboratory Research

MicroRNA-200c Inhibits Epithelial-Mesenchymal Transition by Targeting the BMI-1 Gene Through the Phospho-AKT Pathway in Endometrial Carcinoma Cells In Vitro

Fengling Li12ABCDEF, Aihua Liang2BCDE, Yan Lv3BDE, Guohong Liu2BCDE, Aili Jiang2BCDE, Peishu Liu1ABCDEFG*

Med Sci Monit 2017; 23:5139-5149

Abstract

BACKGROUND: MicroRNA-200c (miR-200c) is a short non-coding RNA that has a role in tumorigenesis and cancer progression. The aims of this study were to investigate the role of miR-200c in cell migration and epithelial-mesenchymal transition (EMT) in endometrial carcinoma cells in vitro.

MATERIAL AND METHODS: Potential direct targets of miR-200c were identified through the TargetScan database. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used study the expression of miR-200c in the endometrial carcinoma cell lines, Ishikawa and JEC, in vitro. Cell migration was studied using transwell assays. Expression of the mesenchymal marker, N-cadherin, the epithelial marker, E-cadherin, the transcription factor, Slug, the BMI-1 protein, AKT, and p-AKT were measured using Western blot. Small interfering RNA (siRNA) was used to silence the BMI-1 gene to study the targeting effect.

RESULTS: Over-expression of miR-200c in Ishikawa and JEC cells resulted in reduced cell migration and proliferation. Western blot showed that overexpression of miR-200c downregulated the expression of the BMI-1 protein, p-AKT, N-cadherin and Slug, and the expression E-cadherin was upregulated; silencing miR-200c reversed these results. Silencing the BMI-1 gene inhibited EMT and suppressed p-AKT in miR-200c-inhibited endometrial carcinoma cells by increasing E-cadherin expression, reducing the expression of N-cadherin and the EMT-associated transcription factor, Slug.

CONCLUSIONS: In endometrial carcinoma cells in vitro, miR-200c inhibited EMT by targeting the BMI-1 gene through the p-AKT pathway.

Keywords: Carcinoma, Endometrioid, Epithelial-mesenchymal transition, Proto-Oncogene Proteins c-akt

0 Comments

This Week Editorial

02 August 2021 : Editorial

Editorial: The National COVID Cohort Collaborative Consortium Combines Population Data with Machine Learning to Evaluate and Predict Risk Factors for the Severity of COVID-19

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.934171

Med Sci Monit 2021; 27:e934171

Coronavirus/Covid 19

30 July 2021 : Clinical Research

Rural and Female Patients with Old Myocardial Infarction Lacked Knowledge and Preventive Measures During th...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.928512  

16 July 2021 : Review article

Silent Hypoxemia in Patients with COVID-19 Pneumonia: A Review

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.930776  

09 July 2021 : Database Analysis

A Simple Clinical Prediction Tool for COVID-19 in Primary Care with Epidemiology: Temperature-Leukocytes-CT...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.931467  

07 July 2021 : Database Analysis

Chest Radiological Findings and Clinical Characteristics of Laboratory-Confirmed COVID-19 Patients from Sau...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.932441  

In Press

03 Aug 2021 : Clinical Research

Comparison of Outcomes Following TiRobot-Assisted Sacroiliac Screw Fixation with Bone Grafting and Traditio...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.932724  

02 Aug 2021 : Database Analysis

RNA N6-Methyladenosine Patterns in Hepatocellular Carcinoma Reveal a Distinct Immune Infiltration Landscape...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.930994  

02 Aug 2021 : Clinical Research

Vitamin D Receptor Is a Sepsis-Susceptibility Gene in Chinese Children

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.932518  

30 Jul 2021 : Clinical Research

Rural and Female Patients with Old Myocardial Infarction Lacked Knowledge and Preventive Measures During th...

Med Sci Monit In Press  ::  DOI: 10.12659/MSM.928512  

Most Viewed

20 Mar 2020 : Clinical Research

Social Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...

DOI :10.12659/MSM.923921

Med Sci Monit 2020; 26:e923921

15 Apr 2020 : Clinical Research

Psychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...

DOI :10.12659/MSM.924171

Med Sci Monit 2020; 26:e924171

05 May 2020 : Review article

An Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development

DOI :10.12659/MSM.924700

Med Sci Monit 2020; 26:e924700

26 Apr 2020 : Clinical Research

Comparison of Prevalence and Associated Factors of Anxiety and Depression Among People Affected by versus P...

DOI :10.12659/MSM.924609

Med Sci Monit 2020; 26:e924609

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750