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17 March 2017 : Laboratory Research  

Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells

Yang Yu1BEF, Lixiang Lin2BC, Yifei Zhou1D, Xiaolang Lu3CE, Xiwen Shao1BD, Chuanlu Lin1EF, Kehe Yu3CD, Xiaolei Zhang1BF, Jianjun Hong1AF*, Ying Chen4AG

DOI: 10.12659/MSM.903892

Med Sci Monit 2017; 23:1334-1339

Abstract

BACKGROUND: Hypoxic conditions play roles in functioning of human tendon-derived stem cells (hTSCs). The goal of this study was to investigate the effect of various hypoxic conditions in self-renewal capacity and differentiation of TSCs.

MATERIAL AND METHODS: hTSCs was obtain from supraspinatus tendon donors. Colony formation and cell proliferation assay were used to assess the self-renewal of hTSCs. qRT-PCT and Western blot analysis were used to examine stemness and multi-differentiation potential of hTSCs.

RESULTS: We found that culturing at 5% O2 is more beneficial for the self-renewal of hTSCs than the other 3 culture conditions, with larger colony size and numbers. The proliferation of hTSCs in 5%, 10%, and 20% O2 cultures increased after seeding. The number of cells in the 5% O2 condition was higher than that in other culture; however, self-renewal capacity of hTSCs in 0.5% O2 was inhibited. The expression levels of stem cell markers, including NS, Nanog, Oct-4, and SSEA-4, were highest in 0.5% O2 culture. Furthermore, hTSCs cultured in 20% O2 exhibited significantly higher expression of the 3 markers (PPAR-γ, Sox-9, and Runx-2).

CONCLUSIONS: Hypoxic condition of culture encouraged self-renewal capacity of hTSCs, but inhibited their multi-differentiation potential, compared to normoxic condition of culture. Moreover, excessively low oxygen concentration impaired the capacity of hTSCs.

Keywords: adult stem cells, Antigens, Differentiation, Cell Hypoxia

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750