Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

03 March 2017 : Laboratory Research

Prolonged Integration Site Selection of a Lentiviral Vector in the Genome of Human Keratinocytes

Wei Qian1ABCDEF, Yong Wang1ABCDEF, Rui-fu Li1BCDF, Xin Zhou1BCF, Jing Liu1BCF, Dai-zhi Peng1ABCDEFG*

DOI: 10.12659/MSM.903094

Med Sci Monit 2017; 23:1116-1122

Abstract

BACKGROUND: Lentiviral vectors have been successfully used for human skin cell gene transfer studies. Defining the selection of integration sites for retroviral vectors in the host genome is crucial in risk assessment analysis of gene therapy. However, genome-wide analyses of lentiviral integration sites in human keratinocytes, especially after prolonged growth, are poorly understood.

MATERIAL AND METHODS: In this study, 874 unique lentiviral vector integration sites in human HaCaT keratinocytes after long-term culture were identified and analyzed with the online tool GTSG-QuickMap and SPSS software.

RESULTS: The data indicated that lentiviral vectors showed integration site preferences for genes and gene-rich regions.

CONCLUSIONS: This study will likely assist in determining the relative risks of the lentiviral vector system and in the design of a safe lentiviral vector system in the gene therapy of skin diseases.

Keywords: Keratinocytes, Lentivirus, Virus Integration

0 Comments

Weekly Editorial

18 October 2021 : Editorial

Editorial: Global Initiatives Support the Use and Regulation of Digital Health Technology During the COVID-19 Pandemic

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.935123

Med Sci Monit 2021; 27:e935123

SARS-CoV-2/COVID-19

20 October 2021 : Editorial

Editorial: The 2021 European Society of Cardiology (ESC) Guidelines on the Real-World Prevention of Atheros...

Med Sci Monit In Press; DOI: 10.12659/MSM.935172  

11 October 2021 : Clinical Research

Clinical Features and Temporal Lung Radiographic Changes in 25 Patients Recovering from COVID-19 Pneumonia:...

Med Sci Monit In Press; DOI: 10.12659/MSM.933381  

08 September 2021 : Clinical Research

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

Med Sci Monit In Press; DOI: 10.12659/MSM.932788  

In Press

21 Oct 2021 : Database Analysis

Construction and Verification of a Hypoxia-Stemness-Based Gene Signature for Risk Stratification in Esophag...

Med Sci Monit In Press; DOI: 10.12659/MSM.934359  

21 Oct 2021 : Database Analysis

MS4A1 as a Potential Independent Prognostic Factor of Breast Cancer Related to Lipid Metabolism and Immune ...

Med Sci Monit In Press; DOI: 10.12659/MSM.934597  

21 Oct 2021 : Clinical Research

Predicting Values of Neutrophil-to-Lymphocyte Ratio (NLR), High-Sensitivity C-Reactive Protein (hs-CRP), an...

Med Sci Monit In Press; DOI: 10.12659/MSM.934569  

20 Oct 2021 : Editorial

Editorial: The 2021 European Society of Cardiology (ESC) Guidelines on the Real-World Prevention of Atheros...

Med Sci Monit In Press; DOI: 10.12659/MSM.935172  

Most Viewed Current Articles

20 Mar 2020 : Clinical Research

Social Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...

DOI :10.12659/MSM.923921

Med Sci Monit 2020; 26:e923921

15 Apr 2020 : Clinical Research

Psychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...

DOI :10.12659/MSM.924171

Med Sci Monit 2020; 26:e924171

05 May 2020 : Review article

An Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development

DOI :10.12659/MSM.924700

Med Sci Monit 2020; 26:e924700

26 Apr 2020 : Clinical Research

Comparison of Prevalence and Associated Factors of Anxiety and Depression Among People Affected by versus P...

DOI :10.12659/MSM.924609

Med Sci Monit 2020; 26:e924609

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750