Antioxidation of Cerium Oxide Nanoparticles to Several Series of Oxidative Damage Related to Type II Diabetes Mellitus In Vitro
Jing-hui Zhai, Yi Wu, Xiao-ying Wang, Yue Cao, Kan Xu, Li Xu, Yi Guo
College of Pharmacy, Jilin University, Changchun, Jilin, China (mainland)
Med Sci Monit 2016; 22:3792-3797
It is well known that cerium oxide nanoparticles (CeNPs) have intense antioxidant activity. The antioxidant property of CeNPs are widely used in different areas of research, but little is known about the oxidative damage of Cu2+ associated with Type II diabetes mellitus (T2DM).
MATERIAL AND METHODS: In our research, the function of CeNPs was tested for its protection of β-cells from the damage of Cu2+ or H2O2. We detected hydroxyl radicals using terephthalic acid assay, hydrogen peroxide using Amplex Ultra Red assay, and cell viability using MTT reduction.
RESULTS: We found that CeNPs can persistently inhibit Cu2+/H2O2 evoked hydroxyl radicals and hydrogen peroxide in oxidative stress of β-cells.
CONCLUSIONS: CeNPs will be useful in developing strategies for the prevention of T2DM.
Keywords: Diabetes Mellitus, Type 2, Hydrogen Peroxide, Islet Amyloid Polypeptide