Effect of Fu-Zheng-Xiao-Liu Granules on Expression of Human Epidermal Growth Factor Receptor 2 (HER-2) and Proliferation and Apoptosis of Breast Cancer Cell Line SKBR-3
Ting Mo, Shuangbing Yue, Huan Tian, Hong Lin, Guanglu Zhang, Zili Zhang
Department of Integrated Chinese and Western Medicine, Shenzhen Second People’s Hospital (Shenzhen University First Affiliated Hospital), Shenzhen, Guangdong, China (mainland)
Med Sci Monit 2016; 22:5068-5073
Previous research showed that granulized Fu-Zheng-Xiao-Liu has a significant effect on breast cancer. However, it remains unclear whether HER-2 plays a role in this anti-cancer effect.
MATERIAL AND METHODS: Serum of male SD rats administered Fu-Zheng-Xiao-Liu granules (SF) was prepared and used to treat HER-2 positive breast cancer cell line SKBR-3. PBS and herceptin were used as negative and positive controls, respectively. MTT was used to detect the proliferation of SKBR-3 cells. Flow cytometry was used to measure the apoptosis of SKBR-3 cells. Western blot and immunofluorescence were used to measure the expression change of HER-2.
RESULTS: Serum of male SD rats administered Fu-Zheng-Xiao-Liu granules had significantly reduced HER-2 expression at both mRNA level and protein level, significantly inhibited proliferation of SKBR-3 cells, and significantly increased apoptosis of SKBR-3 cells, compared to that of the blank control group or serum control group.
CONCLUSIONS: Fu-Zheng-Xiao-Liu granules affect proliferation and apoptosis through inhibition of HER-2 transcription and translation, providing an experimental basis for further study of the mechanism by which Fu-Zheng-Xiao-Liu granules affect breast cancer.
Keywords: Cell Line, Tumor, Breast Neoplasms - pathology, Apoptosis - genetics, Cell Proliferation - drug effects, Cell Survival - genetics, Drugs, Chinese Herbal - pharmacology, Gene Expression Regulation, Neoplastic - drug effects, RNA, Messenger - metabolism, Receptor, erbB-2 - metabolism, Risk Factors