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eISSN: 1643-3750

Experimental Study of Magnetic Multi-Walled Carbon Nanotube-Doxorubicin Conjugate in a Lymph Node Metastatic Model of Breast Cancer

Jian Ji, Minfeng Liu, Yue Meng, Runqi Liu, Yan Yan, Jianyu Dong, Zhaoze Guo, Changsheng Ye

Department of Hepatobiliary Breast Surgrey, The Fifth Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, China (mainland)

Med Sci Monit 2016; 22:2363-2373

DOI: 10.12659/MSM.898597

Available online: 2016-07-07

Published: 2016-07-07


#898597

BACKGROUND: The lymphatic system plays a significant role in the defense of a subject against breast cancer and is one of the major pathways for the metastasis of breast cancer. To improve the prognosis, many means, including surgery, radiotherapy, and chemotherapy, have been used. However, the combination of all these modalities has limited efficacy. Lymph nodes, therefore, have become an exceptionally potential target organ in cancer chemotherapy.
MATERIAL AND METHODS: A lymph node metastatic model of breast cancer was established in BALB/c mice. Magnetic multi-walled carbon nanotube carrier with good adsorption and lymph node-targeting capacity was prepared and conjugated with doxorubicin to make the magnetic multi-walled carbon nanotube-doxorubicin suspension. Dispersions of doxorubicin, magnetic multi-walled carbon nanotube-doxorubicin, and magnetic multi-walled carbon nanotube were injected into lymph node metastatic mice to compare their inhibitory effects on tumor cells in vivo. Inhibition of these dispersions on EMT-6 breast cancer cells was detected via MTT assay in vitro.
RESULTS: Although no significant difference was found between the effects of doxorubicin and magnetic multi-walled carbon nanotube-doxorubicin with the same concentration of doxorubicin on EMT-6 breast cancer cells in vitro, in terms of sizes of metastatic lymph nodes and xenograft tumors, apoptosis in metastatic lymph nodes, and adverse reactions, the magnetic multi-walled carbon nanotube-doxorubicin group differed significantly from the other groups.
CONCLUSIONS: The magnetic multi-walled carbon nanotube-doxorubicin clearly played an inhibitory role in lymph node metastases to EMT-6 breast cancer cells.

Keywords: Apoptosis - drug effects, Antibiotics, Antineoplastic - administration & dosage, Animals, Disease Models, Animal, Doxorubicin - administration & dosage, Drug Delivery Systems - methods, Lymph Nodes - pathology, Magnetics, Mammary Neoplasms, Experimental - pathology, Mice, Mice, Inbred BALB C, Nanotubes, Carbon, Prognosis, Random Allocation



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