Scimago Lab
powered by Scopus
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST


Medical Science Monitor Basic Research


eISSN: 1643-3750

MiR-30a Decreases Multidrug Resistance (MDR) of Gastric Cancer Cells

Chunying Li, Jinhai Zou, Guoqi Zheng, Jiankun Chu

Department of Gastroenterology, Cangzhou Central Hospital, Cangzhou, Hebei, China (mainland)

Med Sci Monit 2016; 22:4509-4515

DOI: 10.12659/MSM.898415

Available online: 2016-11-22

Published: 2016-11-22


BACKGROUND: The effectiveness of chemotherapy for gastric cancer is largely limited by either intrinsic or acquired drug resistance. In this study, we aimed to explore the association between miR-30a dysregulation and multidrug resistance (MDR) in gastric cancer cells.
MATERIAL AND METHODS: We recruited 20 patients with advanced gastric cancer. Chemosensitivity was assessed after completion of the chemotherapy. SGC-7901 and SGC-7901/DDP cells were transfected for miR-30a overexpression or knockdown. Then, MTT assay was performed to assess the IC50 of DPP and 5-FU in SGC-7901 and SGC-7901/DDP cells. Flow cytometry analysis was used to detect DPP- and 5-FU-induced cell apoptosis. Western blot analysis and immunofluorescence staining were used to assess EMT of the cells.
RESULTS: MiR-30a was significantly downregulated in the chemoresistant tissues. In both SGC-7901 and SGC-7901/DDP cells, miR-30a overexpression decreased the expression of P-gp, a MDR-related protein. MTT assay and flow cytometry analysis showed that miR-30a inhibition increased chemoresistance, while miR-30a overexpression decreased chemoresistance in gastric cancer cells. Both Western blot analysis and immunofluorescence staining confirmed that miR-30a inhibition decreased E-cadherin but increased N-cadherin in SGC-7901 cells, while miR-30a overexpression increased E-cadherin but decreased N-cadherin in SGC-7901 cells.
CONCLUSIONS: MiR-30a can decrease multidrug resistance (MDR) of gastric cancer cells. It is also an important miRNA modulating EMT of the cancer cells.

Keywords: Apoptosis - drug effects, Antineoplastic Combined Chemotherapy Protocols - pharmacology, Cadherins - metabolism, Cell Line, Tumor, Cell Proliferation - drug effects, Cisplatin - administration & dosage, Down-Regulation - drug effects, Drug Resistance, Neoplasm - genetics, Epithelial-mesenchymal transition, Fluorouracil - administration & dosage, Gene Knockdown Techniques, MicroRNAs - genetics, Stomach Neoplasms - pathology, Transfection