22 December 2016 : Laboratory Research
Induction of Apoptosis in U937 Cells by Using a Combination of Bortezomib and Low-Intensity UltrasoundTimur Saliev1ADEFG*, Loreto B. Feril Jr.2ABCDE, Koichi Ogawa2BDE, Akiko Watanabe2BDE, Dinara Begimbetova1BDEF, Askhat Molkenov1CD, Dauren Alimbetov1DEF, Katsuro Tachibana2ACDF
Med Sci Monit 2016; 22:5049-5057
BACKGROUND: We scrutinized the feasibility of apoptosis induction in blood cancer cells by means of low-intensity ultrasound and the proteasome inhibitor bortezomib (Velcade).
MATERIAL AND METHODS: Human leukemic monocyte lymphoma U937 cells were subjected to ultrasound in the presence of bortezomib and the echo contrast agent Sonazoid. Two types of acoustic intensity (0.18 W/cm² and 0.05 W/cm²) were used for the experiments. Treated U937 cells were analyzed for viability and levels of early and late apoptosis. In addition, scanning electron microscopy analysis of treated cells was performed.
RESULTS: The percentage of cells that underwent early apoptosis in the group treated with ultrasound and Sonazoid was 8.0±1.31% (intensity 0.18 W/cm²) and 7.0±1.69% (0.05 W/cm²). However, coupling of bortezomib and Sonazoid resulted in an increase in the percentage of cells in the early apoptosis phase, up to 32.50±3.59% (intensity 0.18 W/cm²) and 33.0±4.90% (0.05 W/cm²). The percentage of U937 cells in the late apoptosis stage was not significantly different from that in the group treated with bortezomib only.
CONCLUSIONS: Our findings indicate the feasibility of apoptosis induction in blood cancer cells by using a combination of bortezomib, ultrasound contrast agents, and low-intensity ultrasound.
Keywords: Bortezomib - pharmacology, Cell Survival - drug effects, Microscopy, Electron, Scanning, U937 Cells, Ultrasonics
01 May 2022 : EditorialEditorial: Cardiovascular Complications at One Year After SARS-CoV-2 Infection are Independent of Underlying Cardiovascular Risk Factors or Severity of COVID-19
Med Sci Monit 2022; 28:e937048
04 May 2022 : Clinical ResearchEffects of Wearing Face Masks on Exercise Capacity and Ventilatory Anaerobic Threshold in Healthy Subjects ...
Med Sci Monit In Press; DOI: 10.12659/MSM.936069
22 April 2022 : Clinical ResearchFactors Associated with Falls During Hospitalization for Coronavirus Disease 2019 (COVID-19)
Med Sci Monit In Press; DOI: 10.12659/MSM.936547
27 April 2022 : Meta-AnalysisEffect of the COVID-19 Pandemic on Serum Vitamin D Levels in People Under Age 18 Years: A Systematic Review...
Med Sci Monit In Press; DOI: 10.12659/MSM.935823
19 May 2022 : Clinical ResearchAssociation Between Serum Homocysteine Levels and Severity of Diabetic Kidney Disease in 489 Patients with ...
Med Sci Monit In Press; DOI: 10.12659/MSM.936323
18 May 2022 : Clinical ResearchPhysical and Psychosocial Concept Domains Related to Health-Related Quality of Life (HRQL) in 50 Girls and ...
Med Sci Monit In Press; DOI: 10.12659/MSM.936801
18 May 2022 : Clinical ResearchAssociation Between Variants of the Mannose-Binding Lectin 2 Gene and Susceptibility to Sepsis in the Haina...
Med Sci Monit In Press; DOI: 10.12659/MSM.936134
Most Viewed Current Articles
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Nov 2020 : Review articleLong-Term Respiratory and Neurological Sequelae of COVID-19
Med Sci Monit 2020; 26:e928996