Xiaochuan Wang, Ping Cao, Jian Liu, Peng Du, Zhiqiong Wang, Wei Chen, Chang Liu, Yifei Wu
(Department of Dermatology, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, China (mainland))
Med Sci Monit 2017; 23:46-56
This study aimed to explore whether 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) restrains pathological hyperplasia of fibroblasts from hyperplastic scar tissues, and to investigate the potential mechanism.
MATERIAL AND METHODS: We used MTT assay, flow cytometry, and terminal-deoxynucleotidyl transferase mediated nick-end labeling (TUNEL) to examine the effects of ALA-PDT on proliferation, cell cycle, and apoptosis of fibroblasts isolated from hyperplastic scar tissues. The growth-promoting effect of fibroblasts on vascular endothelial cells was measured by cell co-culture. Real-time PCR and Western blot analysis were performed to detect the expression levels of transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (a-SMA), Collagen I, Collagen III, vascular endothelial growth factor-A (VEGFA), and basic fibroblast growth factor (bFGF).
RESULTS: ALA-PDT inhibited proliferation delayed cell cycle progress, promoted apoptosis of fibroblasts, and suppressed its growth-promoting effect on vascular endothelial cells, and decreased expression of TGF-β1, α-SMA, Collagen I, Collagen III, VEGFA, and bFGF.
CONCLUSIONS: ALA-PDT effectively restrained pathological hyperplasia of fibroblasts from hyperplastic scar tissues, which may provide a research basis for clinical therapy of hyperplastic scars.
Keywords: Apoptosis - drug effects, Aminolevulinic Acid - pharmacology, Adult, Cell Differentiation - drug effects, Cells, Cultured, Cicatrix - pathology, Collagen - metabolism, Female, Fibroblast Growth Factor 2 - metabolism, Fibroblasts - pathology, Humans, Hyperplasia - drug therapy, Male, Photochemotherapy - methods, young adult