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13 June 2016 : Animal Research  

Adenosine Triphosphate (ATP) Inhibits Voltage-Sensitive Potassium Currents in Isolated Hensen’s Cells and Nifedipine Protects Against Noise-Induced Hearing Loss in Guinea Pigs

Rui YeAB, Jun LiuCD, Zhiying JiaDE, Hongyang WangBF, YongAn WangBD, Wei SunAB, Xuan WuBC, Zhifei ZhaoC, Baolong NiuC, Xingqi LiA, Guanghai DaiD, Jianxiong LiFG

DOI: 10.12659/MSM.898150

Med Sci Monit 2016; 22:2006-2012

Abstract

BACKGROUND: There is increasing evidence that adenosine triphosphate (ATP), a well-known neurotransmitter and neuromodulator in the central nervous system, plays an important role as an extracellular chemical messenger in the cochlea.

MATERIAL AND METHODS: Using a whole-cell recording technique, we studied the effects of ATP on isolated Hensen’s cells, which are supporting cells in the cochlea, to determine if they are involved in the transduction of ions with hair cells.

RESULTS: ATP (0.1–10 µM) reduced the potassium current (IK+) in the majority of the recorded Hensen’s cells (21 out of 25 cells). An inward current was also induced by high concentrations of ATP (100 µM to 10 mM), which was reversibly blocked by 100 µM suramin (a purinergic antagonist) and blocked by nifedipine (an L-type calcium channel blocker). After the cochleas were perfused with artificial perilymph solutions containing nifedipine and exposed to noise, the amplitude increase in the compound action potential (CAP) threshold and the reduction in cochlear microphonics was lower than when they were exposed to noise alone.

CONCLUSIONS: Our results suggest that ATP can block IK+ channels at a low concentration and induce an inward Ca2+ current at high concentrations, which is reversed by purinergic receptors. Nifedipine may have a partially protective effect on noise-induced hearing loss (NIHL).

Keywords: Adenosine Triphosphate - pharmacology, Action Potentials - drug effects, Calcium - metabolism, Calcium Channel Blockers - pharmacology, Guinea Pigs, Hair Cells, Auditory - metabolism, Hearing Loss, Noise-Induced - prevention & control, Nifedipine - pharmacology, Organ of Corti, Patch-Clamp Techniques - methods, Potassium Channels, Voltage-Gated - metabolism, Random Allocation, Signal Transduction - physiology, Suramin - pharmacology

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750