17 March 2016 : Laboratory Research
Med Sci Monit 2016; 22:875-879
BACKGROUND: This study aimed to analyze the potential function of lncRNA CCAT2 in cervical cancer cell proliferation and apoptosis.
MATERIAL AND METHODS: Expression level of CCAT2 in cervical cancer cell lines (HeLa, CaSki, and SiHa) was detected by quantitative real-time PCR. CCAT2 knockdown was established by transfecting siRNA into human cervical cancer cells. Its effects on cell proliferation were studied using cell-counting kit-8 assay. The effect of CCAT2 on cervical cancer cells cycle and apoptosis was assessed by flow cytometry assay.
RESULTS: CCK8 assay showed that CCAT2 knockdown inhibited cell proliferation in HeLa, CaSki, and SiHa cells. The flow cytometry confirmed the results that knockdown of CCAT2 could induce cervical cancer cells cycle G1 phase arrestment and trigger the cells apoptosis.
CONCLUSIONS: LncRNA CCAT2 promoted the proliferation and survival of cervical cancer cells.
Keywords: Apoptosis - genetics, Gene Knockdown Techniques, Real-Time Polymerase Chain Reaction, Uterine Cervical Neoplasms - pathology
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