Jianghui Ying, Yunfeng Liu, Ruijin Yang, Yong Zhang, Jianjun Xu
Burn and Wound Healing Centre, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland)
Med Sci Monit 2016; 22:697-701
Available online: 2016-03-01
Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts.
MATERIAL AND METHODS: Quantitative real-time polymerase chain reaction(qRT-PCR) was used to assess the expression of miR-100. Transient transfection of miR-100 mimics and inhibitor was used to up-regulate or down-regulate the expression of miR-100. Cell proliferation and colony formation assay were used to test the cell growth, and wound healing assay and transwell migration assay were used to evaluate the cell migration.
RESULTS: miR-100 expression was notably downregulated in the burn-denatured fibroblasts compared to normal controls. Functionally, transfection of miR-100 inhibitors improved the growth and migration abilities of burn-denatured fibroblasts. In contrast, upregulation of miR-100 inhibits the growth and migration of burn-denatured fibroblasts.
CONCLUSIONS: Based on these observations, we concluded that miR-100 can inhibit the growth and migration of burn-denatured fibroblasts.
Keywords: Cell Movement, Burns - pathology, Fibroblasts - pathology, MicroRNAs - metabolism, Up-Regulation - genetics