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eISSN: 1643-3750

MiR-494 Inhibits Epithelial Ovarian Cancer Growth by Targeting c-Myc

Jialing Yuan, Kana Wang, Mingrong Xi

(Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China (mainland))

Med Sci Monit 2016; 22:617-624

DOI: 10.12659/MSM.897288

Published: 2016-02-24


BACKGROUND: Epithelial ovarian cancer (EOC) is the most lethal malignant gynecological cancer. MicroRNAs (miRNAs) play important roles in the pathogenesis of ovarian cancer. The role of miR-494 in EOC has not been fully investigated.
MATERIAL AND METHODS: MiR-494 levels in ovarian cancer tissues and cells were tested by qRT-PCR. Cells were transfected with miR-494 mimics or miR-494 ASO by Lipofectamine. Bioinformatics algorithms from TargetScanHuman were used to predict the target genes of miR-494. The c-Myc protein level was assayed by Western blot. The interaction between miR-494 and c-Myc was confirmed by dual luciferase assays.
RESULTS: MiR-494 showed low levels in EOC tissues and cells. Overexpression of miR-494 inhibited cell growth and migration of EOC cells and vice versa. c-Myc is the targeted gene of miR-494.
CONCLUSIONS: MiR-494 has an anti-tumor role in EOC via c-Myc.

Keywords: Base Sequence, Aged, Adult, Cell Line, Tumor, Cell Proliferation, Female, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs - metabolism, Middle Aged, Molecular Sequence Data, Neoplasm Invasiveness, Neoplasms, Glandular and Epithelial - pathology, Ovarian Neoplasms - pathology, Proto-Oncogene Proteins c-myc - metabolism



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