Up-Regulation of Angiotensin-Converting Enzyme (ACE) Enhances Cell Proliferation and Predicts Poor Prognosis in Laryngeal Cancer
Chao-dong Han, Wen-sheng Ge
Department of Otolaryngology, Liaocheng People’s Hospital and EENT Hospital, Liaocheng, Shandong, China (mainland)
Med Sci Monit 2016; 22:4132-4138
Available online: 2016-11-01
The angiotensin-converting enzyme (ACE, CD143) gene plays a crucial role in the pathology of many cancers. Previous studies mostly focused on the gene polymorphism, but the other functions of ACE have rarely been reported. The purpose of this study was to investigate the expression of ACE and its biological function, as well as its prognostic value, in laryngeal cancer.
MATERIAL AND METHODS: The expression of ACE was detected by quantitative real-time polymerase chain reaction (qRT-PCR) analysis in 106 patients with laryngeal cancer and 85 healthy people. Then the cell proliferation was estimated after the cell lines Hep-2 were transfected with pGL3-ACE and empty vector, respectively. In addition, the relationship between ACE expression and clinicopathologic characteristics was analyzed. Finally, Kaplan-Meier analysis was used to evaluate the overall survival of patients with different ACE expression, while Cox regression analysis was conducted to reveal the prognostic value of ACE in laryngeal cancer.
RESULTS: Our results demonstrate that ACE is over-expressed in laryngeal cancer and thus promotes cell proliferation. The up-regulation of ACE was significantly influenced by tumor stage and lymph node metastasis. Patients with high ACE expression had a shorter overall survival compared with those with low ACE expression according to Kaplan-Meier analysis. The ACE gene was also found to be an important factor in the prognosis of laryngeal cancer.
CONCLUSIONS: Our study shows that the ACE gene was up-regulated, which promoted the cell proliferation, and it could be an independent prognostic marker in laryngeal cancer.
Keywords: Biomarkers, Tumor - metabolism, Adult, Carcinoma, Squamous Cell - pathology, Case-Control Studies, Cell Line, Tumor, Cell Proliferation - drug effects, Head and Neck Neoplasms - pathology, Kaplan-Meier Estimate, Laryngeal Neoplasms - pathology, Peptidyl-Dipeptidase A - genetics, Prognosis, RNA, Messenger - genetics, Up-Regulation