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Drug Resistance Characteristics and Macrolide-Resistant Mechanisms of Streptococcus pneumoniae in Wenzhou City, China

Dakang Hu, Zheng Sun, Xinhua Luo, Shuangchun Liu, Lianhua Yu, Ying Qu, Jinhong Yang, Jian Yu, Xiangyang Li, Jin Zhang

Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang, China (mainland)

Med Sci Monit 2016; 22:2731-2735

DOI: 10.12659/MSM.896766

Available online:

Published: 2016-08-02

BACKGROUND: Streptococcus pneumoniae (SP) is a Gram-positive, alpha-hemolytic, facultative anaerobic member of the genus Streptococcus. The erythromycin-resistant methylase (erm) gene and macrolide efflux (mef) gene are the 2 main genes that can mediate SP. Transposon (Tn) also plays an important role in the collection and metastasis of the gene. In the present study we investigated the drug resistance characteristics and the macrolide-resistant mechanisms of SP in Wenzhou City, China.
MATERIAL AND METHODS: Sixty-eight strains of SP were isolated from sputum samples of hospitalized children in the Second Affiliated Hospital of Wenzhou Medical University. These strains were analyzed using antimicrobial susceptibility tests to determine their drug resistance to 10 kinds of antibacterials. Macrolide-resistant phenotypes were identified using K-B method. PCR method was used to analyze the erm B gene, mef A gene, and int Tn gene.
RESULTS: Drug resistance rates of 68 strains of SP were 98.5%, 100.0%, 63.2%, 52.9%, 94.1%, 89.7%, 0.0%, 0.0%, 16.2%, and 14.7% for clindamycin, erythromycin, penicillin G, cefotaxime, tetracycline, sulfamethoxazole/trimethoprim, levofloxacin, vancomycin, chloramphenicol, and amoxicillin, respectively. Total detection rates of the erm B gene, mef A gene, and int Tn gene were 98.5%, 91.2%, and 100.0%, respectively.
CONCLUSIONS: SP shows significant multi-drug resistance in Wenzhou City, whereas there is no clinical value of macrolides antibiotics for SP. cMLSB mediated by erm B gene is the most predominant phenotype among macrolide-resistant SP. The int Tn gene may play an important role in horizontal transfer and clonal dissemination of SP drug resistance genes in Wenzhou City.

Keywords: Bacterial Proteins - genetics, Anti-Bacterial Agents - pharmacology, Child, Child, Preschool, China, Drug Resistance, Multiple, Bacterial - genetics, Macrolides - pharmacology, Membrane Proteins - genetics, Methyltransferases - genetics, Microbial Sensitivity Tests, Pneumococcal Infections - microbiology, Serotyping, Streptococcus pneumoniae - isolation & purification