Qijun Zhang, Sheng Zeng, Chengyuan Quan, Xiaopo Lin
Department of Emergency, Pingyang People’s Hospital, Wenzhou, Zhejiang, China (mainland)
Med Sci Monit 2015; 21:3023-3027
Available online: 2015-10-07
The aim of this study was to investigate the potential function of miR-126 in neural stem cells (NSCs).
MATERIAL AND METHODS: Expression level of miR-126 was detected by quantitative real-time PCR (qRT-PCR). MiR-126 overexpression was established by transfecting miR-126 mimics into human NSC lines (HB1.F3 and HB1.A4 cells). Its effects on cell proliferation were studied using cell-counting kit-8 (CCK8) assay, colony formation assays. Flow cytometry was performed to evaluate the effect of miR-126 on cell survival.
RESULTS: CCK8 assay and colony formation assay showed that overexpression of miR-126 promoted cell proliferation and increased colony numbers in HB1.F3 and HB1.A4 cells. The flow cytometry confirmed the results that miR-126 inhibited cell apoptosis.
CONCLUSIONS: MiR-126 promoted the proliferation and survival of NSCs.
Keywords: Brain - physiology, Cell Line, Lentivirus - genetics, MicroRNAs - metabolism, Neural Stem Cells - cytology, Real-Time Polymerase Chain Reaction, Signal Transduction