Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

13 November 2015 : Animal Research  

MicroRNA-137 Negatively Regulates H2O2-Induced Cardiomyocyte Apoptosis Through CDC42

Junnan WangAB, Rihao XuAG, Junduo WuDE, Zhibo LiEF

DOI: 10.12659/MSM.894648

Med Sci Monit 2015; 21:3498-3504

Abstract

BACKGROUND: Oxidative stress, inducing cardiomyocyte apoptosis or myocardial ischemia, is the major denominator of many cardiac diseases. In this study, we intended to explore the regulatory function of microRNA-137 (miR-137) in oxidative stress-induced cardiomyocyte apoptosis.

MATERIAL AND METHODS: Cardiomyocytes were extracted from newborn C57BL/6 mice and cultured in vitro. Apoptosis was induced by H2O2, and evaluated by TUNEL assay. The effect of cardiomyocyte apoptosis on gene expression of miR-137 was evaluated by qRT-PCR. Lentivirus was used to stably down-regulate miR-137, and the subsequent effects of miR-137 down-regulation on cardiomyocyte apoptosis, its targeted gene CDC42, and caspase pathway were evaluated by TUNEL assay, dual-luciferase reporter assay, and Western blot assay, respectively. Finally, CDC42 was down-regulated by siRNA and its effect on miR-137-mediated cardiomyocyte apoptosis protection was examined.

RESULTS: H2O2 induced significant apoptosis and up-regulated miR-137 in cardiomyocytes, whereas lentivirus-mediated miR-137 down-regulation protected against apoptosis. CDC42 was the direct target gene of miR-137 and proteins of CDC42, caspase-3, and caspase-9 were all regulated by miR-137 down-regulation in cardiomyocyte apoptosis. SiRNA-mediated CDC42 down-regulation reversed the protection of miR-137 down-regulation against cardiomyocyte apoptosis.

CONCLUSIONS: Our work demonstrated miR-137 and CDC42 are critical regulators in cardiomyocyte apoptosis. It may help to identify the molecular targets to prevent myocardial injury in human patients.

Keywords: Apoptosis - genetics, Animals, Cells, Cultured, Hydrogen Peroxide - pharmacology, Mice, Mice, Inbred C57BL, Myocardial Ischemia - pathology, Myocytes, Cardiac - metabolism, Oxidative Stress - drug effects, Up-Regulation - drug effects, cdc42 GTP-Binding Protein - metabolism

Comments

Editorial

01 January 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Patients

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

SARS-CoV-2/COVID-19

25 January 2022 : Editorial  

Editorial: The 2022 World Health Organization (WHO) Priority Recommendations and Response to the Omicron Va...

Med Sci Monit In Press; DOI: 10.12659/MSM.936199  

25 January 2022 : Clinical Research  

Experience of the Polish Medical Air Rescue Service During the First Year of the COVID-19 Pandemic and Meas...

Med Sci Monit In Press; DOI: 10.12659/MSM.935474  

11 January 2022 : Clinical Research  

A Remote Questionnaire-Based Study to Compare Alcohol Use in 1030 Final-Year High School Students in Split-...

Med Sci Monit In Press; DOI: 10.12659/MSM.935567  

31 December 2021 : Clinical Research  

A Prospective Observational Study of 42 Patients with COVID-19 infection and a History of Hepatitis C Virus...

Med Sci Monit 2021; 27:e935075

In Press

27 Jan 2022 : Clinical Research  

Retrospective Study of the Association Between Platelet-to-Lymphocyte Ratio in Patients with Acute Coronary...

Med Sci Monit In Press; DOI: 10.12659/MSM.935002  

25 Jan 2022 : Editorial  

Editorial: The 2022 World Health Organization (WHO) Priority Recommendations and Response to the Omicron Va...

Med Sci Monit In Press; DOI: 10.12659/MSM.936199  

25 Jan 2022 : Clinical Research  

Experience of the Polish Medical Air Rescue Service During the First Year of the COVID-19 Pandemic and Meas...

Med Sci Monit In Press; DOI: 10.12659/MSM.935474  

24 Jan 2022 : Clinical Research  

Causes of Lower Respiratory Tract Infections and the Use of Diagnostic Biomarkers in Blood Samples from Chi...

Med Sci Monit In Press; DOI: 10.12659/MSM.934889  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

05 Mar 2020 : Clinical Research  

The Effects of Social Support on Sleep Quality of Medical Staff Treating Patients with Coronavirus Disease ...

DOI :10.12659/MSM.923549

Med Sci Monit 2020; 26:e923549

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750