Haidong Cai, Yu Qiao, Ming Sun, Xueyu Yuan, Qiong Luo, Yuehua Yang, Shidong Yuan, Zhongwei Lv
Department of Nuclear Medicine, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, China (mainland)
Med Sci Monit 2015; 21:2339-2344
Available online: 2015-08-10
To investigate the in vitro inhibitory effects of PEI-RGD/125I-(αv)ASODN (PEI, polyethylenimine; RGD, Arg-Gly-Asp; ASODN, antisense oligodeoxynucleotide) on the growth and invasion of HepG2 cells.
MATERIAL AND METHODS: ASODN of the integrin αv-subunit was marked with 125I and underwent complexation with PEI-RGD, a PEI derivative. Next, PEI-RGD/125I-(αv) ASODN was introduced into HepG2 cells via receptor-mediated transfection, and its inhibition rate on HepG2 cell growth was tested using the methyl thiazolyl tetrazolium (MTT) method. The effects of PEI-RGD/125I-(αv) ASODN on HepG2 cell invasion ability were evaluated using the Boyden chamber assay.
RESULTS: 1) The 125I marking rate of (αv) ASODN was 73.78±4.09%, and the radiochemical purity was 96.68±1.38% (greater than 90% even after a 48-h incubation period at 37°C), indicating high stability. 2) The cytotoxicity assays showed that the cell inhibition rates did not differ significantly between the PEI-RGD/125I-(αv)ASODN group and the PEI-RGD/(αv) ASODN group, but they were both significantly higher than in the other groups and were positively correlated (r=0.879) with the dosage within a certain range. 3) The invasion assays showed that the inhibition rate was significantly greater in the PEI-RGD/125I-(αv) ASODN group compared to the other groups.
CONCLUSIONS: PEI-RGD/125I-(αv) ASODN can efficiently inhibit the growth and proliferation of HepG2 cells and can also weaken their invasive ability.
Keywords: Cell Proliferation - radiation effects, Base Sequence, Hep G2 cells, Integrin alphaV - genetics, Iodine Radioisotopes - administration & dosage, Oligodeoxyribonucleotides, Antisense - genetics, Oligopeptides - administration & dosage, Polyethyleneimine - analogs & derivatives