Up-Regulation of miR-9 Target CBX7 to Regulate Invasion Ability of Bladder Transitional Cell Carcinoma
Dalong Xie, Chao Shang, Hui Zhang, Yan Guo, Xiaojie Tong
Department of Anatomy, College of Basic Medicine, China Medical University, Shenyang, Liaoning, China (mainland)
Med Sci Monit 2015; 21:225-230
Bladder urothelial carcinoma is the most common genitourinary system cancer in China. The objective of this study was to investigate whether the miR-9 can regulate the invasion ability of human bladder transitional cell carcinoma cells by down-regulation of CBX7.
Material and Methods: The expression of miR-9 was detected by quantitative real-time PCR in bladder transitional cell carcinomas (TCC) and normal bladder transitional cell (NBTC) samples. Bioinformatics software was used to predict some potential target genes of miR-9. T24 cells were transfected with pre-miR-9, and the CBX7 protein expression was detected by Western blot. Luciferase activities assay was selected to verify that CBX7 was a direct and specific gene of miR-9. T24 cells were transfected with pcDNA-CBX7, and the expression of CBX7 gene was detected. Then, the transwell assay was used to detect the invasion ability of T24 cells with CBX7 over-expression.
Results: The expression of miR-9 increased significantly in human TCC specimens compared to that in NBTC specimens. TargetScan and PicTar software programs predicted CBX7 gene was a target gene of miR-9. The pre-miR-9 could up-regulate the miR-9 expression and down-regulate CBX7 protein expression. The luciferase activities assay verified that CBX7 gene was a direct and specific target gene of miR-9. The pcDNA-CBX7 transfection could up-regulate the CBX7 protein expression, and the invasion ability of T24 cells with CBX7 over-expression decreased significantly.
Conclusions: Aberrantly expressed miR-9 contributes to T24 cells invasion, partly through directly down-regulating CBX7 protein expression in TCC. This miRNA signature offers a new potential therapeutic target for TCC.
Keywords: Carcinoma, Transitional Cell - metabolism, 3' Untranslated Regions, China, Computational Biology, Down-Regulation, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, MicroRNAs - metabolism, Neoplasm Invasiveness, Polycomb Repressive Complex 1 - metabolism, Up-Regulation, Urinary Bladder - pathology, Urinary Bladder Neoplasms - pathology