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02 June 2015 : Laboratory Research  

Anticancer Effect and Apoptosis Induction of Gambogic Acid in Human Leukemia Cell Line K562 In Vitro

Jinhao ChenABCD, Min ZhouABCD, Qian ZhangBC, Jingyan XuBF, Jian OuyangAEG

DOI: 10.12659/MSM.893004

Med Sci Monit 2015; 21:1604-1610

Abstract

BACKGROUND: The aim of this study was to investigate the anticancer effect and related mechanisms of gambogic acid (GA), a traditional Chinese medicine, on human leukemia cell line K562, together with the effect on bone marrow mononuclear cells (MNCs).

MATERIAL AND METHODS: K562 cells and MNCs were treated with various concentrations and treatment times of GA. Inhibitory rate was detected by use of the Cell Counting Kit-8 (CCK-8) assay. Apoptosis was analyzed by morphological detection, Annexin-V/PI doubling staining, and TUNEL assays. The expression changes of pivotal proteins were evaluated by Western blotting.

RESULTS: GA not only suppressed cell proliferation, but also induced apoptosis of K562 cells in a dose-dependent manner. While it did not significantly inhibit cell proliferation of MNCs, it did induce apoptosis in a dose-dependent manner. CCK-8 assay revealed that the proliferation of K562 cells was significantly inhibited when the concentration of GA was more than 0.5 μM. Morphological detection showed the nuclei became denser and more intense orange in K562 cells after GA treatment compared with the untreated group. The expression levels of BCL-2, nuclear factor-κB (NF-κB), c-myc, phosphatidylinositol3-kinase (PI3K), and phosphorylation of serine-threonine kinase (p-AKT) were down-regulated by GA.

CONCLUSIONS: GA significantly suppressed the proliferation of K562 cells, but has less effect on MNCs. The inhibition of K562 cells proliferation and apoptosis induced by GA might be related to the down-regulation of BCL-2, NF-κB, c-myc, PI3K, and p-AKT.

Keywords: Antineoplastic Agents - pharmacology, Annexin A5, Blotting, Western, Cell Count, Cell Proliferation - drug effects, Gene Expression Regulation, Neoplastic - drug effects, In Situ Nick-End Labeling, In Vitro Techniques, K562 Cells, Leukemia - drug therapy, Phosphatidylinositol 3-Kinases - metabolism, Proto-Oncogene Proteins c-bcl-2 - metabolism, Proto-Oncogene Proteins c-myc - metabolism, Xanthones - pharmacology

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750