miR-630 Overexpression in Hepatocellular Carcinoma Tissues is Positively Correlated with alpha-Fetoprotein
Jian-wen Zhang, Yang Li, Xian-Cheng Zeng, Tong Zhang, Bin-Sheng Fu, Hui-Min Yi, Qi Zhang, Nan Jiang
Department of Hepatic Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China (mainland)
Med Sci Monit 2015; 21:667-673
MicroRNA-630 (miR-630) has been shown to be involved in various human malignancies. However, its role in hepatocellular carcinoma (HCC) remains unknown.
Material and Methods: TaqMan qRT-PCR assay was performed to detect the expression of miR-630 in 42 pairs of HCC tissues and corresponding noncancerous hepatocellular tissues, and its correlations with clinicopathologic features and serum alpha-fetoprotein (AFP) level of patients were analyzed.
Results: The present study found that miR-630 expression was significantly increased in HCC tissues and cells compared with their normal counterparts. miR-630 expression level did not significantly chang at stage I but was markedly increased at advanced TNM stage (stage II~III). In addition, the increased expression of miR-630 in tissues of HCC appeared in patients who exhibited elevated serum levels of AFP (>25 ng/ml), but not in those with normal AFP levels (≤25 ng/ml). The miR-630 expression in carcinoma tissues revealed a positive correlation with the levels of serum alpha-fetoprotein (AFP; R2=0.768).
Conclusions: These results suggest that miR-630 is associated with tumor progression of hepatocellular carcinoma and may be a potential prognosis indicator.
Keywords: Adult, Carcinoma, Hepatocellular - genetics, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Liver Neoplasms - genetics, MicroRNAs - metabolism, Neoplasm Staging, alpha-Fetoproteins - metabolism