Proteomic Profiling of Aging in Glomeruli of Mice by using Two-Dimensional Differential Gel Electrophoresis
Xiaodan Liu, Qiuling Fan, Gang Yang, Lining Wang
Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland)
Med Sci Monit 2015; 21:419-425
Glomerular proteins were analyzed by proteomics to screen proteins participating in maturation of glomeruli before senescence and to find key proteins involved in the aging process.
Material and Methods: Glomeruli of C57BL/6 mice at 8 and 20 weeks were separated by kidney perfusion. Proteomic profiles of glomeruli were investigated by using two-dimensional differential gel electrophoresis and MALDI-TOF mass spectrometry.
Results: We identified 22 differentially expressed proteins. Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice. Out of these 22 proteins, 18% take part in protein transport, protein targeting, and proteolysis; 5% in glycolysis; 14% in transcription; 9% in electron transport; 9% were chaperones; and 9% were hydrolases.
Conclusions: Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging. In this study we found that aging altered the expression of ATP synthase subunit beta. Further studies on this protein might help to understand the mechanism of renal aging.
Keywords: Electrophoresis, Gel, Two-Dimensional, Animals, Aging - metabolism, Gene Expression Regulation, Developmental - genetics, Kidney Glomerulus - metabolism, Mice, Mice, Inbred C57BL, Mitochondrial Proton-Translocating ATPases - metabolism, Proteome - metabolism, Proteomics