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Medical Science Monitor Basic Research


eISSN: 1643-3750

The clinical significance of lymphangiogenesis in renal cell carcinoma

Paweł Dębiński, Janusz Dembowski, Paweł Kowal, Tomasz Szydełko, Anna Kołodziej, Bartosz Małkiewicz, Krzysztof Tupikowski, Romuald Zdrojowy

Med Sci Monit 2013; 19:606-611

DOI: 10.12659/MSM.883981

Available online:

Published: 2013-07-24


Background: The formation of lymphatic vessels (lymphangiogenesis) occurs in tumor tissues and is crucial for tumor development and progression in some cancers. Lymphangiogenesis and its clinical effect on renal cell carcinoma have been less thoroughly investigated in comparison with angiogenesis. The aim of this study was to evaluate the role of lymphangiogenesis as a prognostic factor in renal cell carcinoma (RCC).
Material and Methods: The expression of peritumoral/intratumoral lymphatics was studied by immunohistochemical methods in paraffin-embedded nephrectomy specimens from 133 patients with clear cell carcinoma. Patients were divided into 3 groups depending on postoperative follow-up: I) patients without metastases, II) patients with metastases during follow-up, and III) patients with metastases during the operation. Peritumoral lymphatics (PTL) and intratumoral lymphatics (ITL) were immunostained with a D2-40 antibody.
Results: The mean number of PTL present in each group was I=14.1, II=10.6, III=12.1. The mean number of ITL present in each group was I=0.7, II=2.3, III=2.3. The 3 groups showed statistically significant differences only in the case of ITL. A mean count of ITL ≥1 is significantly associated with an increased risk of regional lymph node involvement and distant metastasis. Patients with expression ITL >0.2 and PTL ≤15.2 had a significantly shorter cancer-specific survival.
Conclusions: The number of ITL showed an association with more aggressive cases of RCC and progression of disease. Therefore, the level of expression ITL, together with stage and histological grading, may provide valuable predictive information about the outcome of treatment.

Keywords: lymphangiogenesis, Lymphatic Vessels - pathology, Kidney Neoplasms - physiopathology, Carcinoma, Renal Cell - physiopathology, Aged, 80 and over, Adult, Poland - epidemiology, Survival Rate