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Platelet glycoprotein IIb/IIIa polymorphism HPA-3 b/b is associated with increased risk of ischemic stroke in patients under 60 years of age

Hao Duan, Yan Cai, Xiaojiang Sun

Med Sci Monit 2012; 18(1): CR19-24

DOI: 10.12659/MSM.882195

Available online: 2011-12-22

Published: 2011-12-22

Background:    The role of genetic risk factors in ischemic stroke is unclear. Platelet glycoprotein IIb/IIIa (GpIIb-IIIa) has been implicated in the pathogenesis of ischemic stroke. We sought to evaluate the relationship between the GpIIb/IIIa complex gene polymorphism and ischemic stroke.
    Material/Methods:    We investigated the association of the GpIIb/IIIa complex gene polymorphism with stroke risk in 306 patients with acute ischemic stroke and 266 control subjects by determining the GpIIb and GpIIIa genotype from leukocyte DNA by polymerase chain reaction (PCR) followed by FokI and ScrFI digestion, respectively.
    Results:    Compared with controls, more patients presented with coronary heart disease, hypertension, smoking history, and diabetes. In addition, the patients had higher levels of cholesterol and glucose compared with the control subjects. All donors in the GpIIIa (n=572) group expressed the GpIIIa PlA1 (HPA-1 aa) phenotype. There were no significant differences between the HPA-3 genotype (GpIIb) patient distribution (aa=39.9%, ab=41.4%, bb=28.7%) and healthy control subjects (aa=36.1%, ab=35.0%, bb=28.9%) (P=0.580). Among study participants <60 years, there was a significant difference in the HPA-3 genotype distributions of patients (aa=42.9%, ab=19.8%, bb=37.4%) and healthy control subjects (aa=43.3%, ab=38.8%, bb=17.9%) (P=0.007). Furthermore, HPA-3 b/b increased the risk of ischemic stroke >2-fold (P=0.008).
    Conclusions:    The GpIIb Ile/Ser843 gene polymorphism is associated with ischemic stroke among young and middle-aged adults (<60 years), especially males. The GpIIIa PlA1 phenotype has no relationship to ischemic stroke.

Keywords: Polymerase Chain Reaction, Platelet Glycoprotein GPIIb-IIIa Complex - genetics, Genotype, Genetic Predisposition to Disease - genetics, DNA Primers - genetics, Cholesterol - blood, Blood Glucose - metabolism, Antigens, Human Platelet - genetics, Polymorphism, Genetic, Risk Factors, Sex Factors, Stroke - genetics