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26 October 2011

Cardiomyopathy in the mouse model of Duchenne muscular dystrophy caused by disordered secretion of vascular endothelial growth factor

Dariusz NowakABCDEFG, Hanna KozlowskaABCDEFG, Jerzy S. GieleckiCDEF, Jan RowinskiABG, Anna ZuradaDEF, Krzysztof GoralczykCD, Wladimir BozilowBG

DOI: 10.12659/MSM.882043

Med Sci Monit 2011; 17(11): BR332-338

Abstract

Background: Duchenne muscular dystrophy (DMD) is a genetic neuromuscular disorder that affects skeletal muscles and cardiac muscle tissue. In some cases, myocardial injury secondary to hypoxia can lead to dilative cardiomyopathy (DCM). A genetic defect in the dystrophin gene may increase the susceptibility of myocardium to hypoxia. Available data suggest that this may be caused by impaired secretion of NO, which is bound with secretion of VEGF-A.
Material/Methods: Male mice C57BI/10ScSn mdx (animal model of DMD) and healthy mice C57BI/10ScSn were exposed to hypobaric hypoxia in low-pressure chambers. Their hearts were harvested immediately after and 1, 3, 7, and 21 days after exposure to hypoxia. Normobaric mice were used as controls. The expression of VEGF-A in myocardium and cardiac vessel walls was evaluated using immunohistochemistry, Western blotting, and in situ hybridization.
Results: VEGF-A expression in myocardium and vessel walls of healthy mice peaked 24 hours after exposure to hypoxia. The expression of VEGF-A in vessel walls was similar in dystrophic and healthy mice; however, VEGF-A expression in the myocardium of dystrophic mice was impaired, peaking around day 7. In the heart, the total level of VEGF depends on VEGF expression in myocardium, not in vessel endothelium, and our research demonstrates that the expression of VEGF is dystrophin-dependent.
Conclusions: Disordered secretion of VEGF-A in hypoxic myocardium caused the total level of this factor to be impaired in the heart. This factor, which in normal situations protect against hypoxia, promotes the gradual progression of cardiomyopathy.

Keywords: Muscular Dystrophy, Duchenne - complications, Myocardium - secretion, Mice, Inbred C57BL, Mice, In Situ Hybridization, Cardiomyopathies - etiology, Blotting, Western, Animals, Anoxia - metabolism, Nitric Oxide - secretion, Vascular Endothelial Growth Factor A - secretion

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