Logo MSM

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo MSM Logo MSM Logo MSM

01 October 2010

Effects of X-ray irradiation on methylation levels of p16, MGMT and APC genes in sporadic colorectal carcinoma and corresponding normal colonic mucosa

Lukasz KrakowczykABDEF, Slawomir BlamekACF, Joanna Katarzyna StrzelczykABDG, Anna PlachetkaFG, Adam MaciejewskiEF, Stanislaw PołtorakBEG, Andrzej WiczkowskiEG

Med Sci Monit 2010; 16(10): CR469-474 :: ID: 881196


Background: Colorectal cancer, one of the most aggressive cancers, occurs with a high incidence world-wide. Cancer development and progression is dictated by a series of alterations in a number genes, e.g., tumor suppressor genes, DNA repair genes, oncogenes and others. DNA methylation is an epigenetic modification that is profoundly altered in most cancers. Radiation is a well-known genotoxic agent and human carcinogen that gives rise to a variety of long-term effects. The inheritance of genomic instability suggests the possible involvement of epigenetic mechanisms, such as changes in methylation of the cytosine residues located within CpG dinucleotides. In the current study we evaluated the effect of X-ray irradiation on methylation levels of p16, MGMT and APC genes in colorectal cancers and normal colonic mucosa.
Material/Methods: Fresh tissue samples were obtained from 28 patients (ages 26 to 81 years) with primary colorectal adenocarcinoma and corresponding normal tissues. We used methylation-specific polymerase chain reaction (MSP) for analysis of the methylation status of p16, MGMT, APC1A and APC1B genes.
Results: Methylation of p16, MGMT, APC1A and APC1B was detected in 42%, 67%, 42% and 20% of tumors before X-ray irradiation and in 63%, 56%, 25% and 31% after radiotherapy, respectively. In corresponding normal colonic mucosa, methylation of p16, MGMT and APC1A was detected in 17%, 25% and 8% before and in 19%, 31% and 6% after irradiation, respectively.
Conclusions: Differences between groups, although noticeable, did not reach the level of statistical significance.

Keywords: Intestinal Mucosa - pathology, Genes, p16, Genes, APC, DNA Methylation - radiation effects, Colorectal Neoplasms - radiotherapy, Colon - pathology, Aged, 80 and over, Adult, O(6)-Methylguanine-DNA Methyltransferase - genetics, X-Rays


Weekly Editorial

27 September 2021 : Editorial

Editorial: Global Regulatory Initiatives Deliver Accelerated Approval of the First Bispecific Therapeutic Monoclonal Antibody for Advanced Non-Small Cell Lung Cancer (NSCLC)

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.934854

Med Sci Monit 2021; 27:e934854


08 September 2021 : Clinical Research

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

Med Sci Monit In Press; DOI: 10.12659/MSM.932788  

16 July 2021 : Review article

Silent Hypoxemia in Patients with COVID-19 Pneumonia: A Review

Med Sci Monit In Press; DOI: 10.12659/MSM.930776  

09 July 2021 : Database Analysis

A Simple Clinical Prediction Tool for COVID-19 in Primary Care with Epidemiology: Temperature-Leukocytes-CT...

Med Sci Monit In Press; DOI: 10.12659/MSM.931467  

In Press

24 Sep 2021 : Clinical Research

A Single-Center Study of a Resin Inlay Dental Implant-Fixed Prosthesis for Closing Proximal Contact Loss in...

Med Sci Monit In Press; DOI: 10.12659/MSM.933809  

24 Sep 2021 : Database Analysis

Relationships Between Obstructive Sleep Apnea and Cardiovascular Disease: A Bibliometric Analysis (2010-2021)

Med Sci Monit In Press; DOI: 10.12659/MSM.933448  

24 Sep 2021 : Database Analysis

A Statistical Prediction Model for Survival After Kidney Transplantation from Deceased Donors

Med Sci Monit In Press; DOI: 10.12659/MSM.933559  

24 Sep 2021 : Clinical Research

Value of Ocular Endoscopy in Extraction of Intraocular Foreign Bodies of Cilia in Patients with Open Ocular...

Med Sci Monit In Press; DOI: 10.12659/MSM.932970  

Most Viewed Current Articles

20 Mar 2020 : Clinical Research

Social Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...

DOI :10.12659/MSM.923921

Med Sci Monit 2020; 26:e923921

15 Apr 2020 : Clinical Research

Psychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...

DOI :10.12659/MSM.924171

Med Sci Monit 2020; 26:e924171

05 May 2020 : Review article

An Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development

DOI :10.12659/MSM.924700

Med Sci Monit 2020; 26:e924700

26 Apr 2020 : Clinical Research

Comparison of Prevalence and Associated Factors of Anxiety and Depression Among People Affected by versus P...

DOI :10.12659/MSM.924609

Med Sci Monit 2020; 26:e924609

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750