Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 June 2010

Low expression of Sp100 in laryngeal cancer: Correlation with cell differentiation

Wei LiBCDE, Chao ShangG, Chao GuanB, Yong ZhangB, Kailai SunF, Weineng FuA

Med Sci Monit 2010; 16(6): BR174-178 :: ID: 880605

Abstract

Background: Sp100 is a permanent ProMyelocytic Leukaemian nuclear bodies (PML NB)-associated protein, and has been reported to participate in the regulation of transcriptional activity, apoptosis and other cellular biological processes. The aim of the present study was to explore the expression of Sp100 and its potential clinical implications in laryngeal cancer.
Material/Methods: The mRNA and protein levels of Sp100 in 96 laryngeal cancer samples and paired normal epithelium were examined by RT-PCR, Western blot and immuno-histochemical staining. The correlation of Sp100 expression with clinicopathological features of these patients was assessed by Chi-squared test.
Results: The coherent low Sp100 expression of both transcriptional and translational levels were confirmed in the malignant tissues compared to the normal mucosa, and the expression was down-regulated among the well-, moderately- and poorly-differentiated cancer cells accordingly. Moreover, immuno-histochemical staining demonstrated that Sp100 showed a predominantly nuclear pattern in well-differentiated cancer cells, and diffuse cellular distribution in cytoplasm in poorly-differentiated cancer cells. Besides histological type of cancer cells, other clinicopathological characteristics, including age, sex, T classification, lymph node metastasis, distant metastasis and clinical stage, showed no significant correlation with Sp100 low expression.
Conclusions: Our finding provides an important clue to further understanding the role of Sp100 in the initiation and progression of tumorgenesis.

Keywords: Laryngeal Neoplasms - metabolism, Immunohistochemistry - methods, Cell Differentiation, Carcinoma, Squamous Cell - metabolism, Blotting, Western, Autoantigens - biosynthesis, Antigens, Nuclear - biosynthesis, Neoplasm Metastasis, Reverse Transcriptase Polymerase Chain Reaction

Comments

Editorial

01 May 2022 : Editorial  

Editorial: Cardiovascular Complications at One Year After SARS-CoV-2 Infection are Independent of Underlying Cardiovascular Risk Factors or Severity of COVID-19

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.937048

Med Sci Monit 2022; 28:e937048

SARS-CoV-2/COVID-19

24 May 2022 : Clinical Research  

Screening for SARS-CoV-2 Infection in Students at the Medical University of Warsaw, Poland Between November...

Med Sci Monit In Press; DOI: 10.12659/MSM.936962  

04 May 2022 : Clinical Research  

Effects of Wearing Face Masks on Exercise Capacity and Ventilatory Anaerobic Threshold in Healthy Subjects ...

Med Sci Monit In Press; DOI: 10.12659/MSM.936069  

22 April 2022 : Clinical Research  

Factors Associated with Falls During Hospitalization for Coronavirus Disease 2019 (COVID-19)

Med Sci Monit In Press; DOI: 10.12659/MSM.936547  

27 April 2022 : Meta-Analysis  

Effect of the COVID-19 Pandemic on Serum Vitamin D Levels in People Under Age 18 Years: A Systematic Review...

Med Sci Monit In Press; DOI: 10.12659/MSM.935823  

In Press

24 May 2022 : Clinical Research  

Screening for SARS-CoV-2 Infection in Students at the Medical University of Warsaw, Poland Between November...

Med Sci Monit In Press; DOI: 10.12659/MSM.936962  

23 May 2022 : Clinical Research  

Fully Automatic Knee Joint Segmentation and Quantitative Analysis for Osteoarthritis from Magnetic Resonanc...

Med Sci Monit In Press; DOI: 10.12659/MSM.936733  

20 May 2022 : Clinical Research  

Efficacy of Focused Extracorporeal Shock Wave Therapy in Chronic Low Back Pain: A Prospective Randomized 3-...

Med Sci Monit In Press; DOI: 10.12659/MSM.936614  

19 May 2022 : Clinical Research  

Association Between Serum Homocysteine Levels and Severity of Diabetic Kidney Disease in 489 Patients with ...

Med Sci Monit In Press; DOI: 10.12659/MSM.936323  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750