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31 August 2009

Octuplet pregnancy following intracytoplasmic sperm injection and cryo embryo transfer

Vanda RepiskaABCDF, Ivan LehockyBCD, Lubos DanisovicDE, Ivan VargaDF, Daniel BohmerAF, Ludovit DanihelCD, Stefan GalbavyACD

Med Sci Monit 2009; 15(9): CS143-147 :: ID: 878166

Abstract

Background
It is well known that the frequency of multiple gestation increases after in vitro fertilization in which more than one embryo is transferred. The aim is to report an octuplet pregnancy following intracytoplasmic sperm injection and cryo embryo transfer.
Material and Method
A 31-year-old woman and her 35-year-old husband, both Caucasian, complained of primary infertility. The intracytoplasmic sperm injection and cryo embryo transfer procedure was recommended as treatment. Ovarian stimulation was performed using recombinant follicle-stimulating hormone and human menopausal gonadotropin. Two embryos were transferred to the uterus. This fertilization cycle was unsuccessful. Three months later, cryo embryo transfer of two frozen/thawed embryos was performed, which resulted in pathological monozygotic octuplet anembryonic pregnancy. Two, and later eight, empty sacs were detected by sonographic examination. Cytogenetic examination revealed normal male karyotype. DNA analysis confirmed identical polymorphisms in all the gestation sacs, i.e. a monozygotic origin of all eight sacs.
Results

Conclusions
We report a rare case of octuplet pregnancy after intracytoplasmic sperm injection and cryo embryo transfer.

Keywords: Pregnancy, Polymorphism, Genetic, Karyotyping, Embryo Transfer, Cryopreservation, Adult, Pregnancy, Multiple, Sperm Injections, Intracytoplasmic

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