Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

30 March 2009

Qa-2 mRNA in peripheral blood mononuclear cells: a potential marker of acute allograft rejection

Nan LuABCDEF, Furong WanAB, Xiaojing YangABD, Chuanxin WangACDE, Zhang YiACDE, Shengmei ZhaoABDE, Shenghua WangABD, Xiaoli LiABD, Xiangdong LiAB, Mingchen ZhuBD, Hong JiangBD, Jinbo FengBD, Xiong ZouADEG

Med Sci Monit 2009; 15(4): BR99-105 :: ID: 869607


HLA-G displays an immunotolerogenic role and its expressions in grafts/sera are related to allograft acceptance. However, it is still unclear how its transcription level in peripheral blood mononuclear cells (PBMCs) changes during allograft rejection. The aim of the present study was to detect the expression changes of the murine homolog of HLA-G, Qa-2, serially during the whole process of graft rejection with mouse skin transplantation models and to investigate their relationship with the pathological grade of graft rejection and immunosuppressive therapy.
Material and Method
Full-thickness skin derived from donor mice (C57BL/6j for syngeneic groups and Balb/c for allogeneic groups) was transplanted onto the dorsal thorax of recipient mice (C57BL/6j). Pathological allograft changes were classified into four grades. Qa-2 mRNA expression of PBMCs was detected serially by real-time RT-PCR.
Qa-2 mRNA did not show any obvious change in the syngeneic recipients without immunosuppressive treatment, inferring that surgical stress might not influence Qa-2 expression. A significant increase in Qa-2 mRNA was observed in the immunosuppressant-treated recipients, suggesting that the increased Qa-2 mRNA level might be attributed to the immunosuppressive treatment. During allograft rejection, the Qa-2 mRNA level decreased significantly, especially with immunosuppressive treatment, and the decreased expression was detected when the allograft presented grade 2-3 pathological rejection, much earlier than when gross rejection was observed.
These results suggest that decreased expression of Qa-2 mRNA in PBMCs may be a potential marker for predicting acute allograft rejection as well as a tool to monitor the effects of immunosuppressive treatment.

Keywords: Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - genetics, Mice, Inbred C57BL, Mice, Mice, Inbred BALB C, Leukocytes, Mononuclear - immunology, Histocompatibility Antigens Class I - genetics, Graft Rejection, DNA Primers, Biological Markers - blood, Base Sequence, Animals, Skin Transplantation, Transplantation, Homologous



01 January 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Patients

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952


11 January 2022 : Clinical Research  

A Remote Questionnaire-Based Study to Compare Alcohol Use in 1030 Final-Year High School Students in Split-...

Med Sci Monit In Press; DOI: 10.12659/MSM.935567  

08 November 2021 : Database Analysis  

Virtual Screening and Molecular Docking to Study the Mechanism of Chinese Medicines in the Treatment of Cor...

Med Sci Monit In Press; DOI: 10.12659/MSM.934102  

31 December 2021 : Clinical Research  

A Prospective Observational Study of 42 Patients with COVID-19 infection and a History of Hepatitis C Virus...

Med Sci Monit 2021; 27:e935075

30 December 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

Med Sci Monit 2021; 27:e935379

In Press

17 Jan 2022 : Clinical Research  

Unveiling the Differences in Biological Properties of Dental Pulp Stem Cells from Normal and Inflamed Pulp:...

Med Sci Monit In Press; DOI: 10.12659/MSM.934511  

14 Jan 2022 : Database Analysis  

Identification of Key Genes Associated with Brain Metastasis from Breast Cancer: A Bioinformatics Analysis

Med Sci Monit In Press; DOI: 10.12659/MSM.935071  

13 Jan 2022 : Review article  

A Review of Current Clinical Concepts in the Pathophysiology, Etiology, Diagnosis, and Management of Hyperc...

Med Sci Monit In Press; DOI: 10.12659/MSM.935821  

13 Jan 2022 : Clinical Research  

Effect of Cerebral Microbleeds on Cognitive Function and Quality of Life in Parkinson Disease

Med Sci Monit In Press; DOI: 10.12659/MSM.935026  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

05 Mar 2020 : Clinical Research  

The Effects of Social Support on Sleep Quality of Medical Staff Treating Patients with Coronavirus Disease ...

DOI :10.12659/MSM.923549

Med Sci Monit 2020; 26:e923549

15 Apr 2020 : Clinical Research  

Psychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...

DOI :10.12659/MSM.924171

Med Sci Monit 2020; 26:e924171

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750