01 November 2007
Med Sci Monit 2007; 13(11): BR231-239 :: ID: 512817
Background: Heat shock protein HYOU1, alternatively known as Orp150, plays an important role in hypoxia/ischemia and angiogenesis. Preliminary studies demonstrated increased HYOU1/Orp150 expression in prostate, bladder and invasive breast cancer. This study further evaluates HYOU1/Orp150 expression in different stages of breast cancer such as benign, pre-malignant and malignant lesions, and correlates it with clinical and pathological data.
Material/Methods: Sixty-six paraffin-embedded breast tissue sections were stained by immunohistochemistry using HYOU1/Orp150 antibody. Antibody staining was reviewed and scored 0 (lowest) to 3+ (highest). Clinico-pathological data was compared with HYOU1/Orp150 staining using ANOVA or Student’s t-Test as appropriate with significance determined by P<0.05.
Results: There was a small but significant increase (P<0.01) in HYOU1/Orp150 expression as the tumor transformed from benign (mean 1.52±0.12) to ductal carcinoma in situ (mean 2.05±0.14) and invasive carcinoma (mean 2.1±0.17). HYOU1/Orp150 was partially lost in the benign tissue adjacent to purely invasive cancer (0.88±0.22) and it was less (P=0.046) than what was found in benign only lesions (1.52±0.12) or in benign components adjacent to DCIS (1.47±0.16). Overexpression of HYOU1/Orp150 was associated with tumor lymph node involvement, presence of lymphovascular invasion, and absence of estrogen receptor expression (P<0.01). A trend was observed between HYOU1/Orp150 and nuclear p53 overexpression (P<0.04), but no correlation was detected with age, TNM stage, PR status, HER2/neu or Ki67.
Conclusions: HYOU1/Orp150 is overexpressed in some invasive breast cancers and its overexpression appears to be associated with indicators of poor prognosis. Its association with factors such as lymphovascular invasion and regional nodal involvement suggests a possible role for HYOU1/Orp150 in metastasis.
Keywords: Breast Diseases - metabolism, Proteins - metabolism, Prognosis, Neoplasm Staging, Carcinoma, Intraductal, Noninfiltrating - metabolism, Breast Neoplasms - pathology, Breast Neoplasms - pathology, Proteins - metabolism, Prognosis, Neoplasm Staging, Carcinoma, Intraductal, Noninfiltrating - metabolism, Breast Diseases - metabolism
01 December 2021 : EditorialEditorial: SARS-CoV-2 Vaccine Responses and Breakthrough COVID-19
Med Sci Monit 2021; 27:e935624
03 December 2021 : Laboratory ResearchInfluenza and Influenza-Like Respiratory Virus Infections in Children During the 2019/20 Influenza Season a...
Med Sci Monit In Press; DOI: 10.12659/MSM.934862
08 November 2021 : Database AnalysisVirtual Screening and Molecular Docking to Study the Mechanism of Chinese Medicines in the Treatment of Cor...
Med Sci Monit In Press; DOI: 10.12659/MSM.934102
01 November 2021 : EditorialEditorial: What Can be Learned from National and International Vaccine Adverse Event Reporting Systems Duri...
Med Sci Monit 2022; 28:e935299
06 Dec 2021 : Animal ResearchAstragalus Flavone Induces Proliferation and Differentiation of Neural Stem Cells in a Cerebral Infarction ...
Med Sci Monit In Press; DOI: 10.12659/MSM.933830
06 Dec 2021 : Clinical ResearchAlteration of Serum Metabolites in Women of Reproductive Age with Chronic Constipation
Med Sci Monit In Press; DOI: 10.12659/MSM.934117
06 Dec 2021 : Clinical ResearchBody Composition Changes and Related Factors in Patients with Ulcerative Colitis: A Retrospective Single-Ce...
Med Sci Monit In Press; DOI: 10.12659/MSM.933942
Most Viewed Current Articles
20 Mar 2020 : Clinical ResearchSocial Capital and Sleep Quality in Individuals Who Self-Isolated for 14 Days During the Coronavirus Diseas...
Med Sci Monit 2020; 26:e923921
15 Apr 2020 : Clinical ResearchPsychological Impact and Coping Strategies of Frontline Medical Staff in Hunan Between January and March 20...
Med Sci Monit 2020; 26:e924171
05 May 2020 : Review articleAn Evidence Based Perspective on mRNA-SARS-CoV-2 Vaccine Development
Med Sci Monit 2020; 26:e924700