Med Sci Monit 2000; 6(4): BR668-674 :: ID: 508115
Background: A number of observations suggest that vitamin D plays an important role in maintaining normal cardiovascular function, through its receptors in cardiac muscle or in aortal smooth muscle cells (SMCs). The purpose of this study was to determine the effect of vitamin D active metabolite - calcitriol [1,25(OH)2D3/sub>] on aortal SMCs.
Material/Methods: The cells were isolated from the aortal media of newborn rats by enzymatic digestion and maintained for 6 weeks in primary culture. 1.2 µM of calcitriol was added to the medium every second day.
Results: Light microscopic investigations revealed that the control SMCs formed multilayer with characteristic 'hills and valleys'. Uniform cellular growth patterns were observed after calcitriol treatment. Ultrastructural studies indicated more rapid modulation of SMCs from the 'contractile' to the 'synthetic' phenotype following calcitriol treatment. Elastic fibers were more abundant in treated than in control cultures. The scanning electron microscope showed an increase of regular microvilli on the surface of SMCs. Morphometrical analysis of nuclei demonstrated phenotypic heterogeneity in populations of aortal SMCs. These results suggest that 1) calcitriol can induce changes in the phenotype and in the growth pattern of aortal SMCs, which may be associated with the onset or progression of the atherosclerosis process, 2) the basis of phenotypic changes is related to the stimulation of proliferative activity in these cells.
Keywords: electron microscopy, atherosclerosis, Calcitriol, Culture
01 May 2022 : EditorialEditorial: Cardiovascular Complications at One Year After SARS-CoV-2 Infection are Independent of Underlying Cardiovascular Risk Factors or Severity of COVID-19
Med Sci Monit 2022; 28:e937048
04 May 2022 : Clinical ResearchEffects of Wearing Face Masks on Exercise Capacity and Ventilatory Anaerobic Threshold in Healthy Subjects ...
Med Sci Monit In Press; DOI: 10.12659/MSM.936069
22 April 2022 : Clinical ResearchFactors Associated with Falls During Hospitalization for Coronavirus Disease 2019 (COVID-19)
Med Sci Monit In Press; DOI: 10.12659/MSM.936547
17 May 2022 : Clinical ResearchClinical Application of C-TIRADS Category and Contrast-Enhanced Ultrasound in Differential Diagnosis of Sol...
Med Sci Monit In Press; DOI: 10.12659/MSM.936368
17 May 2022 : Laboratory ResearchArtificial Dermal Scaffold Loaded with Platelet-Rich Plasma Promotes Wound Healing in Pigs by Favoring Angi...
Med Sci Monit In Press; DOI: 10.12659/MSM.936186
16 May 2022 : Clinical ResearchEarly Enteral Nutrition Can Reduce Incidence of Postoperative Hydrocephalus in Patients with Severe Hyperte...
Med Sci Monit In Press; DOI: 10.12659/MSM.935850
Most Viewed Current Articles
30 Dec 2021 : Clinical ResearchRetrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...
Med Sci Monit 2021; 27:e935379
08 Mar 2022 : Review articleA Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...
Med Sci Monit 2022; 28:e936292
01 Nov 2020 : Review articleLong-Term Respiratory and Neurological Sequelae of COVID-19
Med Sci Monit 2020; 26:e928996