01 March 1999
Neutralising effect of an extract from neutrophils (NE) on stimulating influence of LPS E.coli and Intralipid on metabolic activity of cells in the NBT reduction test
Ewa Pawłowska, Gabriel TurowskiMed Sci Monit 1999; 5(2): BR186-192 :: ID: 505073
Abstract
The effect of neutrophil extract (NE) was investigated on the model of cytochemical reaction of peripheral blood neutrophils in NBT reduction tests. The experiments were performed in standard conditions of stimulated reduction test NBTst, using the following factors for neutrophil stimulation: NE, LPS E. coli, IntralipidR (ILP) as well as NE+LPS, NE+ILP and NE+LPS+ILP. The index of differences of NBT-positive cells after stimulation and without stimulation was also used for the interpretation of results (NBTst - NBTnst). Stimulating effect of LPS, ILP and LPS+ILP on the results of NBTst test was confirmed. Differences in the results in relation to NBTnst results were highly statistically significant. Prepared neutrophil extract (NE) proved active and it inhibited the effect of both endotoxin E. coli and IntralipidR in performed experiments in the doses of 4.0 µg LPS and 2.0 mg ILP. Observed differences were highly statistically significant. Neutrophil extract alone (NE) had two effects. It stimulated suspension of neutrophils isolated from 5 different subjects for increased NBT salt reduction. The difference in relation to control NBTnst test were highly statistically significant (t = 5.82, p<0.0043). In the case of neutrophil suspensions from 5 other persons, the effect of NE was different and the differences were not significant (t = Ð2.34). The distribution of the results of NBT reduction tests as a consequence of the use of NE+LPS, NE+ILP and NE+LPS+ILP on neutrophil suspension applied in the tests indicated their different abilities for the response to stimulation. On the basis of the results obtained in our experiments, it may be suggested that neutralising effect of peripheral blood neutrophils in relation to endotoxins of Gram-negative bacilli is the main function of these cells in septic states. Intensified leukocytosis of peripheral blood also seems to confirm this claim. Pathomechanism of these phenomena is still a subject of discussion.
Keywords: neutrophils activity, neutrophil extract, LPS E. coli, Intralipid, NBT reduction test
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
06 Mar 2024 : Clinical Research
Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...Med Sci Monit In Press; DOI: 10.12659/MSM.941102
06 Mar 2024 : Clinical Research
Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...Med Sci Monit In Press; DOI: 10.12659/MSM.943884
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952