01 July 1999
Detection of the SRY (fragment I ) gene as evidence of coitus interruptus in alleged sexual assaults and during molestationMaria Rydzewska, Jerzy Janica, Jadwiga Jaruzelska, Witold Pepiński, Małgorzata Skawrońska
Med Sci Monit 1999; 5(4): BR652-655 :: ID: 503466
We evaluated the molecular assay which may serve as a potential criterion to confirm sexual contact regardless the presence of semen components. The assay is based on the detection of male specific SRY gene sequences obtained from vaginal smears. The SRY gene was amplified by the polymerase chain reaction (PCR) in DNA isolated from cellular debris. We also compared the results of conventional sperm detection methods.
Keywords: PCR, SRY, Diagnosis, Coitus Interruptus
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