Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 March 1998

Late expession of the HLA-DR and HLA-ABC antigens in the posttransplanted myocardium as evidence of chronic rejection

Romuald Wojnicz, Marian Zembala, Michał Zakliczyński, Jerzy Nożyński, Jerzy Foremny, Jacek Wojarski, Danuta Muszko, M. Marius Rozek

Med Sci Monit 1998; 4(2): CR263-267 :: ID: 502598

Abstract

The up-regulation of human leukocyte antigens (HLA) class I and class II, has been suspected of involvement in chronic rejections following orthotopic heart transplantations (OHT). A total of forty four endomyocardial biopsy specimens from 11 patients were immunohistochemically analyzed at intervals of 7±2, 30±4, 84±10 and 340±40 days following OHT. The immunohistological study was based on cryostat sections using monoclonal mouse anti-human HLA antibodies. To detect bounding primary antibodies, the DAKO LSAB¨+ Kit/Alkaline Phosphatase detection system was used. For the assessment of the intensity of staining, a semiquantitative scale of 0-3 was employed. HLA class I (ABC) and class II (DR) staining ³2+ was clinically significant. The study group consisted of patients with ischemic cardiomyopathy (six patients) and dilated cardiomyopathy (five patients) as an indication for OHT. The mean donor ischemia time was 116 minutes (ranging between 45 and 194). None of the patients died during the 14 months observation period. Histological rejection(³2) during the time of the immunohistological study was seen in 2 cases at 7±2 days, in 5 cases at 30±4 days, in 3 cases at 84±10 days and in 1 patient at 340±40 days following OHT. In all endomyocardial biopsies, a late expression (300 days post OHT) of HLA-ABC and HLA-DR occurred as moderate (1/11) and severe (10/11). A significant expression of HLA was only seen in one patient at 7± days following OHT, (7±, vs 300±, p=0.005). This expression had a strong diffuse character and was associated with histological signs of an acute injury. Generally, in contrast to focal and vascular staining during the early period, late staining was diffuse in distribution, including sarcolemmic myocyte staining without histologic signs of myocyte injury. In addition, HLA-DR staining was more intense in capillary endothelial cells as opposed to HLA-ABC, which predominated on myocytes. Late up-regulation of HLA in the myocardium may reflect the time-dependent chronic rejection, without histological signs of a myocardial injury. Our results confirm the hypothesis that the microvascular expression of HLA during an acute rejection may differ from that seen in the chronic state.

Keywords: orthotopic heart transplantation, immunohistology, HLA molecules

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750