Soluble HLA-A and HLA-B antigens in children with congenital adrenal hyperplasia (CAH) and the classic form of 21-hydroxylase deficiency
Gabriel Turowski, Danuta Gieracka-Pażucha, Dorota Turowska-Heydel, Jacek J Pietrzyk
Med Sci Monit 1998; 4(4): CR633-639
Available online: 1998-07-01
It seems important to recognize the function and role of soluble histocompatibility antigens in the pathogenesis of a disease. This especially concerns HLA antigen concentration levels (sHLA-1) in their different allogenic expression. We have tried to determine the phenotypic sHLA-1 concentration levels in 48 children with CAH (the classic form of 21-hydroxylase deficiency). In the first stage of the experiment we determined the appearance of CAH HLA-A, B, C lymphocyte antigens in comparison with 1152 healthy individuals. We noted a statistically high titer of HLA-B47, B61 (40), A3 antigens in CAH children and a lower frequency occurance for HLA- A24 (9) and Bw 6 antigens. We then determined the sHLA-I concentration levels from the serum of CAH children and the results obtained were presented as a total number of points for the inhibition of the cytotoxic reaction as well as the relative 'A' value and the sHLA ÐI concentration levels in 1 ml of serum. SHLA-I concentration levels obtained from healthy individuals served as comparison analysis. SHLA-I concentration levels in CAH children are shown in tables III-VI. Soluble HLA-I antigens from the serum of CAH children demonstrate incorrect concentration level values in comparison with sHLA-I antigens of healthy individuals.
Keywords: congenital adrenal hyperplasia, Pathology, soluble HLA antigens, HLA-ABC antigens frequency