Application of a chemiluminescent labelled single locus probe in the detection of repetetive nucleotide sequences on the human 6-th chromosome
Laszlo Keresztury, Andras Laszik, Maria Penzes, Hargita Hegyesi, Andras Falus
Med Sci Monit 1998; 4(4): BR583-586
Available online: 1998-07-01
Variation in DNA sequence is ideally assayed by direct nucleotide sequencing of a gene region or other homologous genome segment. An easier and cheaper approach, however if variants are analysed by hybridization technology using restriction fragment length polymorphisms (RFLPs) or by detection of the number of tandem repeats (VNTR) of short DNA segments(minisatellites). This study describes results of DNA analysis in demonstrating the application of a chemiluminescent labeled identity kit. The allele frequency distribution of polymorphic DNA sequences has been determined in unrelated individuals. The isolated genomic DNA was cut by means of a Pst I restriction enzyme, size fractionated on agarose gel and hybridized with a chemiluminescent labeled D6 S132 probe. At this locus the Pst I cleaved DNA fragments range between 1841 and 6098 base pairs (bp). A specific genetic pattern was characterised by more frequent fragments (3313 and 3884 bp), and by the rarely occuring ones which are clustered between 1841-2595 and 5227-6098 bp. Our study provides a further possibility for the characterization of individual genomic patterns.
Keywords: Forensic Medicine, gene technology, RFLP, VNTR, chemiluminescent probe