01 September 1997
Effects of interaction between cadmium and selenium on hepatic metabolism in mice. Part II: enzymatic activity and ultrastructure
Mariusz Skowerski, Kazimierz Czechowicz, Janusz Konecki, Krzysztof JasikMed Sci Monit 1997; 3(5): BR648-653 :: ID: 501533
Abstract
The liver is the organ in the body where most toxic substances are metabolized. The histochemical methods of determining enzyme acitivity have provided new interesting information on the metabolism of hepatocytes in Cd, Se and Cd-Se intoxication. The activites of the following enzymes were assayed in the mouse hepatocyte: succinate dehydrogenase(SDH), Mg2+-adenosine triphosphate (Mg2+-ATP-ase), alkaline phosphatase (AP) and acid phosphatase (AcP). Qualitative methods were used in the study. Moreover,ultrastructure of mouse hepatocytes under the experimental conditions was studied.Three-month long experiments were conducted on 24 male white Balby mice. The animals were divided into four groups. The control group I (C) was fed a standard Murigran diet. Group II (Cd.) received 50 ppm Cd as cadmium acetate in drinking water. Group III (Se) was fed a diet supplemented with 5.0 mg Se/kg DM/24 h. as acid sodium selenate. Mice in group IV /Cd+Se/ received a diet supplemented with Cd and Se in the same amounts as Groups II and III. During the experimental period mice were intoxicated with 0.2. mg Se/kg b.w./24h and 16.8 mg Cd/ kg b.w./24h. Our experiments indicated that prolonged Cd intoxication singnificantly lowered the activity of SDH and Mg2+-ATP-ase, which are involved in oxidative phosphorylation and active membrane transport. Accordingly, it seems that energetic processes were deranged. Selenium did not affect enzymatic activities. Se in interaction with Cd suppressed the toxic effects on the level of SDH and Mg2+-ATP-ase activities. Mouse hepatocytes after Cd, Se and Cd-Se-interaction treatment demonstrated ultrastructure changes which appeared to correlate with the enzymatic activities noted and with the previously described incorporation of the protein synthesis precursor. We concluded that prolonged Cd intoxication significantly decreased the activity of enzymes involved in oxidative phosphoralation and active transport. Se in interaction with Cd suppressed toxic effects on SDH and Mg2+-ATP-ase activity.
Keywords: Cadmium, hepatocyte ultrastructure, enzymatic activity, Selenium, Liver
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
06 Mar 2024 : Clinical Research
Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...Med Sci Monit In Press; DOI: 10.12659/MSM.941102
06 Mar 2024 : Clinical Research
Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...Med Sci Monit In Press; DOI: 10.12659/MSM.943884
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952