01 May 1997
5' NC region of the HCV genome complexity detected by SSCP and RFLP analysis
Ilona Bednarek, Urszula Mazurek, Ewa Moric, Oksana Kowalczuk, Dorota Domal-Kwiatkowska, Adam Wilczok, Edyta Czechowska, Ewa Laskowska-Lepiarczyk, Zbigniew Gonciarz, Tadeusz WilczokMed Sci Monit 1997; 3(3): BR328-335 :: ID: 501072
Abstract
Comparison between different HCV isolates from different host pointed to the region-depended variability of HCV. There are several advantages in using the 5' NC region of the HCV genome for genotyping and reflecting quasispecies nature of the HCV. The aim of the presented study was to establish the degree of complexity of 5' NC region HCV genome and grouping of hepatitis C isolates by restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP) by the use of reverse - transcription - polymerase chain reaction products (RT-PCR). Representatives were checked by line probe assay. Isolated RNA preparations from whole blood or serum samples obtained from 55 patients with chronic HCV infection were used for cDNA synthesis. The nested polymerase chain reaction, RFLP experiments, SSCP analysis, the line probe assay and the phylogenetic analysis have shown that the applied methods RFLP and SSCP applied simultaneously to the analysis of the 5' NC virus region provided good evidence, that the region is highly complex. This phenomenon was examined by INNO-LIPA HCV II tests, used normally for genotyping, and it was shown, that the applied RFLP and SSCP methods alone are sufficient to describe the heterogeneity of this region.
Keywords: hepatitis C virus (HCV), Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP), genetic heterogeneit
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
05 Mar 2024 : Clinical Research
Role of Critical Shoulder Angle in Degenerative Type Rotator Cuff Tears: A Turkish Cohort StudyMed Sci Monit In Press; DOI: 10.12659/MSM.943703
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952