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Apoptosis and proliferation of mesangial cells isolated from kidneys undergoing compensatory growth following contralateral nephrectomy: Role of the renin-angiotensin system

Joshua Weissgarten, Sylvia Berman, Shai Efrati, Micha Rapoport, Mirel Cohn, David Modai, Zhan Averbukh

Med Sci Monit 2007; 13(1): BR16-23

ID: 470140

Available online: 2006-12-22

Published: 2006-12-22

Background: Contralateral nephrectomy stimulates compensatory growth of the remaining kidney. Intensive growth is frequently associated with increased apoptosis. The proliferation and apoptosis of cultured
rat mesangial cells isolated from the remaining kidney following contralateral nephrectomy were evaluated. The involvement of the renin-angiotensin system was concomitantly assessed.
Material/Methods: Apoptosis was assessed by TUNEL assay and hematoxylin staining, proliferation by 3H-thymidine incorporation, angiotensin-II (A-II) production by RIA, bradykinin synthesis by specifi c EIA, and AT-1/AT-2 receptor mRNA expression by RT-PCR. 125I-A-II labeling was applied for AT-1/AT-2 receptor density evaluation.
Results: Apoptosis of unstimulated control cultures progressively increased from a baseline of 2.02±0.55% to 8.3±1.19% in 30-min and 12.8±4.11% in 24-h cultures (p

Keywords: Cells, Cultured, Renin-Angiotensin System - drug effects, Receptor, Angiotensin, Type 1 - metabolism, Rats, Sprague-Dawley, Rats, RNA, Messenger - metabolism, Nephrectomy, Mesangial Cells - drug effects, Gene Expression Regulation - drug effects, Angiotensin II Type 2 Receptor Blockers, Cell Separation, Cell Proliferation - drug effects, Captopril - pharmacology, Bradykinin - biosynthesis, Apoptosis - drug effects, Animals, Angiotensin I - metabolism, Angiotensin II - pharmacology