01 November 2006
Leptin increases proliferation of human osteosarcoma cells through activation of PI(3)-K and MAPK pathwaysBartolome Burguera, Amy Brunetto, Adolfo Garcia-Ocana, Roseta Teijeiro, James Esplen, Thierry Thomas, Marta E. Couce, Allan Zhao
Med Sci Monit 2006; 12(11): BR341-349 :: ID: 462552
BACKGROUND: Serum leptin levels are strongly and directly related to fat body mass (FBM). Bone mineral density(BMD) increases with FBM, and obesity has a protective effect against osteoporosis. We have previously demonstrated that leptin therapy has a signifi cant effect in preventing ovariectomy-induced
MATERIAL/METHODS: Saos-2 cell lines were used. Leptin receptor common form (OB-Ra) and long form (OB-Rb) were detected by RT-PCR and immunocytochemistry. PI(3)-K activity was immunoprecipitated using antibodies
RESULTS: In this study, we tested the hypothesis that leptin might be a mediator linking obesity and bone
CONCLUSIONS: Our data support the hypothesis that leptin may increase bone mass by stimulating osteoblast proliferation through activation of the PI(3)-K and MAPK signaling pathways.
Keywords: Cell Line, Tumor, Bone and Bones - metabolism, Enzyme Activation, Enzyme Inhibitors - pharmacology, Insulin Receptor Substrate Proteins, Leptin - physiology, MAP Kinase Signaling System, Osteosarcoma - pathology, Phosphatidylinositol 3-Kinases - metabolism, Phosphoproteins - metabolism, Phosphorylation, Signal Transduction, Tyrosine - chemistry
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