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01 August 2006

ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy

Agnieszka Jezierska, Wojciech Matysiak, Tomasz Motyl

Med Sci Monit 2006; 12(8): BR263-273 :: ID: 452847

Abstract

Background:Activated leukocyte cell adhesion molecule (ALCAM/CD166) is a 105-kDa transmembrane glycoprotein linked with cell migration and development and with cancer progression (malignant melanoma, prostate cancer and, very recently, breast cancer). This report is the first evaluation of ALCAM/CD166 in an estrogen-dependent (MCF-7) and a metastatic (MDA-MB-231) breast cancer cell line and a reference breast cell line (HBL-100). The effects of estrogen and anti-estrogen treatment, bcl-2 overexpression, and ALCAM gene silencing on ALCAM/CD166 protein concentration and cell survival were investigated.

Material/Methods: Laser scanning cytometry, confocal microscopy, and Western blotting were used for the determination of ALCAM/CD166 protein and biochemical markers of apoptosis and autophagy.

Results: 17-β-estradiol increased and tamoxifen inhibited ALCAM/CD166 expression and survival of MCF-7 cells. Overexpression of the bcl2 gene in MCF-7 bcl2/neo, MDA-MB-231 bcl2/neo, and HBL-100 bcl2/neo cells significantly increased ALCAM/CD166 expression and was accompanied by decreasing MMP-2 concentrations. Appearance of the ALCAM/CD166 protein was noted in HBL-100 bcl2/neo in contrast to an almost undetectable level in HBL-100 cells. ALCAM gene silencing in MCF-7 cells decreased the concentration of BCL-2 and increased levels of apoptosis (89-kDa PARP, active caspase7) and autophagy (MAP1LC3, Beclin1) markers.

Conclusions:The above results indicate that ALCAM-ALCAM interactions are crucial to the survival and primary site maintenance of breast cancer cells. Impaired expression of ALCAM/CD166 is associated with the induction of two types of programmed cell death, apoptosis and autophagy, in breast cancer cells. This adhesion molecule can therefore be regarded as a potential novel breast cancer indicator and therapeutic target.

Keywords: Gene Expression, Tumor Markers, Biological, Tumor Cells, Cultured, Tamoxifen - pharmacology, RNA, Messenger - metabolism, Matrix Metalloproteinase 2 - metabolism, Genes, bcl-2 - genetics, Gene Silencing, Gene Expression Regulation, Neoplastic - drug effects, Estradiol - pharmacology, Cell Survival - drug effects, Cell Proliferation - drug effects, Breast Neoplasms - pathology, Activated-Leukocyte Cell Adhesion Molecule - metabolism, Antineoplastic Agents - pharmacology

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